Transgenic plants compromising nucleic acid molecules encoding RAR 1 disease resistance proteins and uses thereof

Abstract

The present invention pertains to transgenic plants comprising nucleic acid molecules comprising nucleotide sequences that encode RAR1 proteins involved in disease resistance, and the RAR1 polypeptides. The invention particularly relates to methods of altering the expressing nucleic acid molecules encoding RAR1 proteins in transgenic plants to alter the level disease resistance, and to transgenic plants, progeny and seed therefrom, having altered enhanced disease resistance. The invention further relates to methods of enhancing expression of R resistance genes, disease resistance signal transduction genes, genes involved in mediating disease resistance or involved in the synthesis of molecules mediating disease resistance. The invention also relates to methods of regulating the expression of other coding sequences of interest by increasing the expression of the nucleic acid molecules of the invention.

1 . A transgenic plant comprising an isolated nucleic acid molecule comprising: 
 a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2;    b) a nucleotide sequence complementary to (a); or    c) a nucleotide sequence which is the reverse complement of (a) or (b).    
     
     
         2 . The transgenic plant according to  claim 1 , wherein said nucleic acid molecule is operably linked to a promoter.  
     
     
         3 . Progeny and seed from the transgenic plant of  claim 1 .  
     
     
         4 . The transgenic plant of  claim 1 , wherein the nucleic acid molecule is expressed in the tissue of the epidermis, vascular tissue, meristem, cambium, cortex, pith, leaf, sheath, root, flower, developing ovule or seed.  
     
     
         5 . The transgenic plant of  claim 1 , wherein the plant is selected from the group consisting of: rice, wheat, barley, rye, corn, potato, canola, soybean, sunflower, carrot, sweet potato, sugarbeet, bean, pea, chicory, lettuce, cabbage, cauliflower, broccoli, turnip, radish, spinach, asparagus, onion, garlic, eggplant, pepper, celery, squash, pumpkin, cucumber, apple, pear, quince, melon, plum, cherry, peach, nectarine, apricot, strawberry, grape, raspberry, blackberry, pineapple, avocado, papaya, mango, banana, soybean, tobacco, tomato, sorghum and sugarcane.  
     
     
         6 . The transgenic plant of  claim 5 , wherein the plant is rice.  
     
     
         7 . The transgenic plant of  claim 1 , wherein the plant is a monocot.  
     
     
         8 . The transgenic plant of  claim 7 , wherein the monocot is selected from the group consisting of L maize, wheat, barley, oats, rye, millet, sorghum, trticale, secale, einkorn, spelt, emmer, teff, milo, flax, gramma grass,  Tripsacum , and teosinte.  
     
     
         9 . The transgenic plant according to  claim 1 , wherein the said nucleotide sequence comprises: 
 (a) the nucleotide sequence of SEQ ID NO: 1;    (b) a nucleotide sequence complementary to (a); or    (c) a nucleotide sequence which is the reverse complement of (a) or (b).    
     
     
         10 . Progeny and seed from the transgenic plant of  claim 9 .  
     
     
         11 . A method of enhancing pathogen or disease resistance in a plant, comprising expressing an expression cassette comprising a RAR1 encoding nucleic acid molecule or an expression cassette comprising SEQ ID NO: 1 in the plant.  
     
     
         12 . The method of  claim 11 , wherein the pathogen or disease is a nematode, bacteria, fungus, virus or viroid.  
     
     
         13 . The method of  claim 12 , wherein the disease is selected from the group consisting of:  Xanthomonas  spp.,  Psudomonas  spp.,  Rhizoctonia  spp.,  Magnaporthe  spp.,  Pythium  spp.,  Phytophthora  spp.,  Fusarium  spp.  Sclerotinia  spp.  
     
     
         14 . A plant produced by the method of  claim 11 , wherein the plant has enhanced pathogen or disease resistance.  
     
     
         15 . A method of increasing expression of R disease resistance genes in a plant, comprising the step of expressing an expression cassette comprising a RAR1 encoding nucleic acid molecule or an expression cassette comprising SEQ ID NO: 1 in the plant.