Antibodies against PAK5

Abstract

The present invention relates to the nucleic acid molecules encoding an STE20-related family of novel protein kinases, ZC1, ZC2, ZC3, ZC4, STLK2, STLK3, STLK4, STLK5, STLK6, STLK7, KHS2, SULU1, SULU3, GEK2, PAK4 and PAK5, segments and domains thereof, as well as various methods useful for the diagnosis and treatment of various kinase-related diseases and conditions. Mammalian nucleic acid molecules encoding these kinases are particularly disclosed, and more specifically human sources of these nucleic acids are disclosed.

1 . An antibody or an antigen-binding fragment thereof having specific binding affinity to an epitope of a PAK5 kinase polypeptide.  
     
     
         2 . An antibody or an antigen-binding fragment thereof according to  claim 1 , wherein said antibody is a monoclonal antibody.  
     
     
         3 . An antibody or an antigen-binding fragment thereof according to  claim 1 , wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 30.  
     
     
         4 . An antibody or an antigen-binding fragment thereof according to  claim 3 , wherein said antibody is a monoclonal antibody.  
     
     
         5 . A hybridoma producing the antibody or an antigen-binding fragment thereof of  claim 1 .  
     
     
         6 . A hybridoma producing the antibody or an antigen-binding fragment thereof of  claim 3 .  
     
     
         7 . A method of detecting a PAK5 kinase polypeptide or a PAK5 kinase polypeptide domain fragment in a sample comprising: (a) contacting said sample with the antibody or antigen-binding fragment thereof of  claim 1  under conditions such that immunocomplexes form, and (b) detecting the presence of said antibody or antigen-binding fragment thereof bound to said PAK5 kinase polypeptide.  
     
     
         8 . A method of detecting a PAK5 kinase polypeptide or a PAK5 kinase polypeptide domain fragment in a sample comprising: (a) contacting said sample with the antibody or antigen-binding fragment thereof of  claim 3  under conditions such that immunocomplexes form, and (b) detecting the presence of said antibody or antigen-binding fragment thereof bound to said PAK5 kinase polypeptide.  
     
     
         9 . A PAK5 detection kit comprising: (a) a first container means containing the antibody or antigen-binding fragment thereof of  claim 1  and (b) a second container means containing a labeled binding partner of said antibody or antigen-binding fragment thereof.  
     
     
         10 . The PAK5 detection kit of  claim 9  further comprising at least one additional container means comprising one or more of the group consisting of wash reagents and reagents capable of detecting the presence of bound antibodies.  
     
     
         11 . A PAK5 detection kit comprising: (a) a first container means containing the antibody or antigen-binding fragment thereof of  claim 3  and (b) a second container means containing a labeled binding partner of said antibody or antigen-binding fragment thereof.  
     
     
         12 . The PAK5 detection kit of  claim 11  further comprising at least one additional container means comprising one or more of the group consisting of wash reagents and reagents capable of detecting the presence of bound antibodies.