Bioadhesive composition comprising a polyphenolic protein

Abstract

A bioadhesive composition comprises a bioadhesive polyphenolic protein derived from a byssus-forming mussel and a polymer-comprising carbohydrate group, but does not include enzymatic oxidizing agents or chemical cross-linking agents.

1 . A bioadhesive composition comprising: 
 a bioadhesive polyphenolic protein derived from a byssus-forming mussel, said protein comprising 30-300 amino acids and consisting essentially of tandemly linked peptide repeats comprising 3-15 amino acid residues, wherein at least 5% of the amino acid residues of said bioadhesive polyphenolic protein are L-3,4-dihydroxyphenyalanine; and    at least one polymer-comprising carbohydrate group,    wherein said bioadhesive composition does not comprise enzymatic oxidizing agents or chemical cross-linking agents.    
     
     
         2 . A composition according to  claim 1 , wherein said at least one polymer-comprising carbohydrate group can form a combined preparation with said bioadhesive polyphenolic protein.  
     
     
         3 . A composition according to  claim 1 , wherein said at least one polymer-comprising carbohydrate group is selected from the group consisting of heparin, chondroitin sulphate, chitosan and hyaluronan.  
     
     
         4 . A composition according to  claim 1 , further comprising pharmaceutically acceptable fine filaments.  
     
     
         5 . A composition according to  claim 1 , wherein 6-25% of the amino acid residues of said bioadhesive polyphenolic protein are L-3,4-dihrdroxyphenyalanine.  
     
     
         6 . A composition according to  claim 1 , further comprising a non-enzymatic oxidizing agent.  
     
     
         7 . A composition according to  claim 6 , wherein said non-enzymatic oxidizing agent is selected from the group consisting of hydrogen peroxide, nitroprusside ions and periodate ions.  
     
     
         8 . A composition according to  claim 1 , further comprising a filler protein.  
     
     
         9 . A composition according to  claim 8 , wherein said filler protein is selected from the group consisting of collagen, casein, albumin, elastin, fibronectin and fibrin.  
     
     
         10 . A combined bioadhesive composition, comprising: 
 a bioadhesive polyphenolic protein derived from a byssus-forming mussel, said protein comprising 3-300 amino acids and consisting essentially of tandemly linked peptide repeats comprising 3-15 amino acid residues, wherein at least 5% of the amino acid residues of said bioadhesive polyphenolic protein are L-3,4-dihrdroxyphenyalanine; and    at least one polymer-comprising carbohydrate group,    wherein said protein and said polymer are in the form of a combined bioadhesive preparation for simultaneous medical use; and    said preparation does not comprise any enzymatic oxidizing agent or chemical cross-linking agent.    
     
     
         11 . A composition according to  claim 10 , further comprising a non-enzymatic oxidizing agent  
     
     
         12 . A composition according to  claim 10 , wherein said non-enzymatic oxidizing agent is selected from the group consisting of hydrogen peroxide, nitroprusside ions and periodate ions.  
     
     
         13 . A composition according to  claim 10 , further comprising pharmaceutically acceptable fine filaments.  
     
     
         14 . A kit, comprising: 
 a combined bioadhesive composition according to  claim 10;  and    instructions for use.    
     
     
         15 . A kit according to  claim 14 , wherein said instructions are instructions for covering or attaching structures to eye tissue.