Biological process for colour reduction of pulp and paper effluent

Abstract

The present invention relates to a bacterium strain of accession no. MTCC 5099, a process for the preparation of innoculum of the said strain, and a process for the reduction of colour from pulp mill effluent using the above said innoculum, which comprises steps of inoculating appropriate aliquots of the pulp and paper mill effluent with the cell slurry obtained, incubating the mixture at about 37° C. at about 100 rpm for time duration ranging between 24-48 hours, assessing colour and total lignin levels to determine the colour removal efficiency of the above said bacterium

1 . A bacterium strain of Accession No. MTCC 5099.  
     
     
         2 . A strain as claimed in  claim 1 , wherein the strain is gram -ve.  
     
     
         3 . A strain as claimed in  claim 1 , wherein the strain is short rods.  
     
     
         4 . A process for the preparation of inoculum of the strain of  claim 1 , said process comprised of: 
 a) isolating a bacterium from activated sludge collected from the effluent treatment plant of a pult and paper mill,    b) culturing said bacterium on nutrient agar media containing 0.1% w/v each of lignin, tannin and vanillin to get pure cultures,    c) inoculating said bacterium in nutrient broth containing 0.01% Tween 80 to obtain starter culture,    d) culturing said bacterium for obtaining required biomass by inoculating an appropriate aliquot of nutrient broth, with the starter culture and incubating the above medium at 37° C./100 rpm for 16-18 hours,    e) Centrifuging the resulting culture, after attaining an aptical density of 1.5-2.0, to obtain a pellet, washing the collected pellet by dissolving in PO 4   −3  buffer, 0.05M, pH 6.8, recentrifuging the pellet, and    f) collecting the pellet obtained from step (e), dissolving in 10 ml of PO 4   −3  buffer, 0.05M, pH 6.8, to obtain cell slurry for teratability studies.    
     
     
         5 . A process as claimed in  claim 4 , wherein the inoculum for using in colour reduction experiments is obtained by inoculating said bacterium in nutrient broth containing 0.01% Tween 80 to obtain starter culture.  
     
     
         6 . A process as claimed in  claim 4 , wherein the above said starter culture is used for obtaining the required inoculum by inoculating an appropriate aliquot of nutrient broth, with the starter culture and incubating the above medium at 37° C./100 rpm for 16-18 hours.  
     
     
         7 . A process as claimed in  claim 4 , wherein the resultant culture is centrifuged for a period of 20 minutes at 4° C.  
     
     
         8 . A process as claimed in  claim 4 , wherein the resultant pellet is washed by dissolving in PO 4   −3  buffer, 0.5M, pH 6.8 and recentrifuging the pellet.  
     
     
         9 . A process as claimed in  claim 4 , wherein the resultant pellet is dissolved in 10 ml of PO 4   −3  buffer, 0.5M, pH 6.8, to obtain inoculum for colour reduction studies.  
     
     
         10 - 16 . (canceled)