Method for quantitatively determining a specific component in a biological specimen, and reagent for quantitative determination

Abstract

A method for quantitatively determining a specific component in a biological specimen, which includes reacting a biological specimen, in the presence of an electron acceptor, with an enzyme which has an ability, by the dehydrogenation reaction, to oxidize the specific component or a substance derived from the specific component, and measuring the formed reductant of the electron acceptor, wherein the method avoids the influence of hemoglobin effectively if any contained in the specimen by using a measuring reagent containing albumin, thereby making an quantitative determination of the target component accurately; and a reagent for the quantitative determination.

1 . A method for quantitatively determining a specific component in a biological specimen, which comprises reacting the biological specimen, in the presence of an electron acceptor, with an enzyme which has an ability, by the dehydrogenation reaction, to oxidize the specific component or a substance derived from the specific component and measuring the formed reductant of the electron acceptor, wherein a measuring reagent containing albumin is used.  
     
     
         2 . The method for quantitatively determining a specific component in a biological specimen according to  claim 1 , wherein the albumin is derived from human or bovine.  
     
     
         3 . The method for quantitatively determining a specific component in a biological specimen according to  claim 1 , wherein a color developer is used for the measurement of the reductant of the electron acceptor.  
     
     
         4 . The method for quantitatively determining a specific component in a biological specimen according to  claim 3 , wherein the color developer is a tetrazolium salt.  
     
     
         5 . The method for quantitatively determining a specific component in a biological specimen according to  claim 1 , wherein the reductant of the electron acceptor is NADH or NADPH.  
     
     
         6 . A reagent for quantitatively determining a specific component in a biological specimen, which comprises albumin, an electron acceptor, and an enzyme which has an ability, by the dehydrogenation reaction, to oxidize the specific component or a substance derived from the specific component.  
     
     
         7 . The reagent for quantitatively determining a specific component in a biological specimen according to  claim 6 , wherein the albumin is derived from human or bovine.  
     
     
         8 . The reagent for quantitatively determining a specific component in a biological specimen according to  claim 6 , which further comprises a color developer.  
     
     
         9 . The reagent for quantitatively determining a specific component in a biological specimen according to  claim 8 , wherein the color developer is a tetrazolium salt.  
     
     
         10 . The reagent for quantitatively determining a specific component in a biological specimen according to  claim 6 , wherein the electron acceptor is NAD or NADP.