Novel serine protease BSSP2

Abstract

There are provided proteins having the amino acid sequences represented by SEQ ID NOS: 2, 4, 6, 8 and 10; proteins having amino acid sequences derived from these amino acid sequences by deletion, substitution or addition of one to several amino acids; and nucleotide sequences encoding the same; transgenic non-human animals with altered expression level of a serine protease BSSP2; an antibody against BSSP2; and a method for detecting BSSP2 in a specimen by using the antibody.

1 . An antibody against a protein selected from the group consisting of: 
 (i) a protein having the amino acid sequence of 238 amino acids represented by SEQ ID NO: 2, a protein having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by SEQ ID NO: 2, or a modified derivative thereof;    (ii) a protein having the amino acid sequence of 273 amino acids represented by SEQ ID NO: 4, a protein having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 4 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by SEQ ID NO: 4, or a modified derivative thereof;    (iii) a protein having the amino acid sequence of 311 amino acids represented by SEQ ID NO: 6, a protein having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 6 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by SEQ ID NO: 6, or a modified derivative thereof;    (iv) a protein having the amino acid sequence of 455 amino acids represented by SEQ ID NO: 8, a protein having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 8 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by SEQ ID NO: 8, or a modified derivative thereof;    (v) a protein having the amino acid sequence of 240 amino acids represented by the 1st to 240th amino acids of SEQ ID NO: 10, a protein having an amino acid sequence derived from the amino acid sequence represented by the 1st to 240th amino acids of SEQ ID NO: 10 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by the 1st to 240th amino acids of SEQ ID NO: 10, or a modified derivative thereof;    (vi) a protein having the amino acid sequence of 457 amino acids represented by the −217th to 240th amino acids of SEQ ID NO: 10, a protein having an amino acid sequence derived from the amino acid sequence represented by the −217th to 240th amino acids of SEQ ID NO: 10 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by the −217th to 240th amino acids of SEQ ID NO: 10, or a modified derivative thereof; and    (vii) a protein having the amino acid sequence of 217 amino acids represented by the −217th to −1st amino acids of SEQ ID NO: 10, a protein having an amino acid sequence derived from the amino acid sequence represented by the −217th to −1st amino acids of SEQ ID NO: 10 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by the −217th to −1st amino acids of SEQ ID NO: 10, or a modified derivative thereof, or a fragment of said protein.    
     
     
         2 . The antibody of  claim 1  which is a polyclonal antibody, a monoclonal antibody or a peptide antibody.  
     
     
         3 . The antibody of  claim 1  which is against the protein having the amino acid sequence of 238 amino acids represented by SEQ ID NO: 2, a protein having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 2 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by SEQ ID NO: 2, or a modified derivative thereof.  
     
     
         4 . The antibody of  claim 1  which is against the protein having the amino acid sequence of 273 amino acids represented by SEQ ID NO: 4, a protein having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 4 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by SEQ ID NO: 4, or a modified derivative thereof.  
     
     
         5 . The antibody of  claim 1  which is against the protein having the amino acid sequence of 311 amino acids represented by SEQ ID NO: 6, a protein having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 6 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by SEQ ID NO: 6, or a modified derivative thereof.  
     
     
         6 . The antibody of  claim 1  which is against the protein having the amino acid sequence of 455 amino acids represented by SEQ ID NO: 8, a protein having an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 8 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by SEQ ID NO: 8, or a modified derivative thereof;  
     
     
         7 . The antibody of  claim 1  which is against the protein having the amino acid sequence of 240 amino acids represented by the 1st to 240th amino acids of SEQ ID NO: 10, a protein having an amino acid sequence derived from the amino acid sequence represented by the 1st to 240th amino acids of SEQ ID NO: 10 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by the 1st to 240th amino acids of SEQ ID NO: 10, or a modified derivative thereof.  
     
     
         8 . The antibody of  claim 1  which is against the protein having the amino acid sequence of 457 amino acids represented by the −217th to 240th amino acids of SEQ ID NO: 10, a protein having an amino acid sequence derived from the amino acid sequence represented by the −217th to 240th amino acids of SEQ ID NO: 10 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by the −217th to 240th amino acids of SEQ ID NO: 10, or a modified derivative thereof.  
     
     
         9 . The antibody of  claim 1  which is against the protein having the amino acid sequence of 217 amino acids represented by the −217th to −1st amino acids of SEQ ID NO: 10, a protein having an amino acid sequence derived from the amino acid sequence represented by the −217th to −1st amino acids of SEQ ID NO: 10 by deletion, substitution or addition of one to several amino acids and having the same property as that of the protein having the amino acid sequence represented by the −217th to −1st amino acids of SEQ ID NO: 10, or a modified derivative thereof, or a fragment of said protein.