Attaching substances to microorganisms

Abstract

The invention relates to surface display of proteins on microorganisms via the targeting and anchoring of heterologous proteins to the outer surface of cells such as yeast, fungi, mammalian, plant cells, and bacteria. The invention provides a proteinaceous substance comprising a reactive group and at least one attaching peptide including a stretch of amino acids having a sequence corresponding to at least a part of the consensus amino acid sequence listed in FIG. 10 and further includes a method for attaching a proteinaceous substance to the cell wall of a microorganism comprising the use of the attaching peptide.

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         22 . A proteinaceous substance comprising: 
 at least one stretch of amino acids derived from a first microorganism, wherein the proteinaceous substance is capable of attaching to a cell wall of a second microorganism, said stretch of amino acids comprising at least one AcmA repeat or a derivative thereof, and    a reactive group selected from the group consisting of antibiotics, hormones, aromatic substances, reporter molecules, antigenic determinants, enzymes, single-chain antibodies or fragments thereof, polyhistidyl tags, and fluorescing proteins.    
     
     
         23 . The proteinaceous substance of  claim 22  wherein said stretch of amino acids has a sequence corresponding to a sequence selected from the group consisting of SEQ ID NO:20 through SEQ ID NO:110, or a homologous sequence derived from another species.  
     
     
         24 . The proteinaceous substance of  claim 22  wherein said second microorganism is a non-recombinant microorganism.  
     
     
         25 . The proteinaceous substance of  claim 24  wherein said second microorganism is selected from the group consisting of gram-positive bacteria and gram-negative bacteria.  
     
     
         26 . A pharmaceutical composition comprising the proteinaceous substance of  claim 22 .  
     
     
         27 . A vaccine comprising the proteinaceous substance of  claim 22 .  
     
     
         28 . A composition that attaches to a bacterial cell wall, said composition comprising: 
 an amino acid sequence comprising at least one AcmA repeat, and, associated therewith, a reactive group.