Methods of applying a biological composition to an individual

Abstract

Provided are methods of applying biological compositions, that is, autologous bioadhesive sealant compositions containing one or more biological agents, to an individual, wherein all the blood components used in preparing the composition are derived from the patient who is to receive the biological composition. In one embodiment, the method comprises obtaining a whole blood sample from an individual; forming an inactive platelet rich plasma from the whole blood sample; mixing a biological agent into the inactive platelet rich plasma; obtaining thrombin from the whole blood sample; mixing the thrombin into the inactive platelet rich plasma to form a biological composition; and applying the biological composition to the individual.

1 - 173 . (canceled)  
     
     
         174 . A method of producing an autologous biological composition comprising: 
 removing blood from an individual to whom the biological composition is intended to be applied;    centrifuging the blood to form a first portion of plasma and a second portion of plasma;    adding a chemical agent to the first portion of plasma;    forming thrombin within the first portion of plasma, wherein the chemical agent participates in the formation of thrombin;    obtaining thrombin from the first portion of plasma; and    mixing the thrombin from the first portion of plasma with the second portion of plasma to produce the autologous biological composition.    
     
     
         175 . The method of  claim 174  wherein the first portion of plasma is platelet poor plasma.  
     
     
         176 . The method of  claim 174  wherein the first portion of plasma is platelet rich plasma.  
     
     
         177 . The method of  claim 174  wherein the second portion of plasma is platelet poor plasma.  
     
     
         178 . The method of  claim 174  wherein the second portion of plasma is platelet rich plasma.  
     
     
         179 . The method of  claim 174  wherein the blood contains a calcium chelating agent.  
     
     
         180 . The method of  claim 179  wherein the calcium chelating agent is citrate.  
     
     
         181 . The method of  claim 174  wherein the chemical agent is calcium ions.  
     
     
         182 . The method of  claim 174  wherein the blood contains heparin.  
     
     
         183 . The method of  claim 182  wherein heparinase is added to the first portion of plasma prior to obtaining the thrombin.  
     
     
         184 . The method of  claim 174  wherein the step of obtaining thrombin from the first portion of plasma includes obtaining a serum comprising the thrombin.  
     
     
         185 . The method of  claim 174  wherein the step of obtaining thrombin from the first portion of plasma comprises the formation of a clot.  
     
     
         186 . The method of  claim 185  wherein the step of obtaining thrombin from the first portion of plasma further comprises triturating the clot to obtain the thrombin.  
     
     
         187 . The method of  claim 174  wherein the step of obtaining thrombin from the first portion of plasma comprises activation of at least a portion of the coagulation pathway.  
     
     
         188 . The method of  claim 174  wherein the step of obtaining thrombin from the first portion of plasma includes passing thrombin through a filter.  
     
     
         189 . The method of  claim 174  wherein the step of obtaining thrombin from the first portion of plasma comprises the conversion of prothrombin to thrombin.  
     
     
         190 . The method of  claim 174  wherein the chemical agent is an activator that activates blood coagulation.  
     
     
         191 . The method of  claim 190  wherein the chemical activator is kaolin.  
     
     
         192 . The method of  claim 174  further comprising the step of placing the first portion of plasma in a container comprising a surface that activates blood coagulation prior to the step of obtaining thrombin from the first portion of plasma.  
     
     
         193 . The method of  claim 174  further comprising the step of placing the first portion of plasma in a container comprising glass prior to the step of obtaining thrombin from the first portion of plasma.  
     
     
         194 . The method of  claim 174  wherein the chemical agent is a restoration agent.  
     
     
         195 . The method of  claim 194  wherein the restoration agent is a calcium salt.  
     
     
         196 . The method of  claim 174  wherein the chemical agent is a clotting activator.  
     
     
         197 . The method of  claim 174  wherein the chemical agent is a contact activator.  
     
     
         198 . The method of  claim 174  wherein the chemical agent is thromboplastin.  
     
     
         199 . The method of  claim 174  further comprising the step of adding a second agent to the second portion of plasma.  
     
     
         200 . The method of  claim 199  wherein the second agent has a biological activity.  
     
     
         201 . The method of  claim 199  wherein the second agent is a drug.  
     
     
         202 . The method of  claim 199  wherein the second agent is selected from the group consisting of: 
 analgesic compounds, antibacterial compounds, antibiotics, antifungal compounds, anti-inflammatories, antiparasitic compounds, antiviral compounds, enzymes, enzyme inhibitors, glycoproteins, growth factors, hormones, steroids, glucocorticosteroids, immunomodulators, immunoglobulins, minerals, neuroleptics, proteins, peptides, lipoproteins, tumoricidal compounds, tumorstatic compounds, toxins and vitamins.    
     
     
         203 . The method of  claim 199  wherein the second agent is selected from the group consisting of growth factors, lymphokines and cytokines.  
     
     
         204 . The method of  claim 199  wherein the second agent is selected from the group consisting of antibacterial compounds, bacteriocidal compounds and bacteriostatic compounds.  
     
     
         205 . The method of  claim 199  wherein the second agent is selected from the group consisting of antibiotics, adriamycin, erythromycin, gentimycin, penicillin and tobramycin.  
     
     
         206 . The method of  claim 199  wherein the second agent is a vitamin.  
     
     
         207 . The method of  claim 206  wherein the vitamin is selected from the group consisting of Vitamin A, Vitamin E, Vitamin B, Vitamin C and Vitamin D.  
     
     
         208 . The method of  claim 199  wherein the second agent is cells.  
     
     
         209 . The method of  claim 208  wherein the cells are bone cells.  
     
     
         210 . The method of  claim 199  wherein the second agent is calcium ions.  
     
     
         211 . The method of  claim 174  further comprising the step of applying the biological composition to the individual.  
     
     
         212 . The method of  claim 211  wherein the step of applying the biological composition to the individual results in a barrier against loss of blood from a vessel.  
     
     
         213 . The method of  claim 211  wherein the step of applying the biological composition to the individual results in a scaffold for repair of tissue.  
     
     
         214 . The method of  claim 211  wherein the step of applying the biological composition to the individual includes applying the biological composition to a needle hole.  
     
     
         215 . The method of  claim 211  wherein the step of applying the biological composition to the individual includes applying the biological composition to an arterial tear.  
     
     
         216 . The method of  claim 211  wherein the step of applying the biological composition to the individual includes applying the biological composition to tissue.  
     
     
         217 . The method of  claim 211  wherein the step of applying the biological composition to the individual includes applying the biological composition to bone.  
     
     
         218 . The method of  claim 211  wherein the step of applying the biological composition to the individual includes applying the biological composition to a nerve.  
     
     
         219 . The method of  claim 211  wherein the step of applying the biological composition to the individual includes applying the biological composition to a vessel.  
     
     
         220 . The method of  claim 211  wherein the step of applying the biological composition to the individual includes applying the biological composition to a wound.  
     
     
         221 . A method of producing an autologous biological composition comprising: 
 removing blood from an individual to whom the biological composition is intended to be applied;    centrifuging the blood to form a first portion of plasma and a second portion of plasma;    adding calcium ions to the first portion of plasma;    forming thrombin within the first portion of plasma via the conversion of prothrombin to thrombin;    obtaining a filtered serum comprising the thrombin from the first portion of plasma; and    mixing the filtered serum comprising the thrombin with the second portion of plasma to produce the autologous biological composition.    
     
     
         222 . The method of  claim 221  wherein the first portion of plasma is platelet poor plasma and the second portion of plasma is platelet poor plasma.  
     
     
         223 . The method of  claim 221  wherein the first portion of plasma is platelet rich plasma and the second portion of plasma is platelet rich plasma.  
     
     
         224 . The method of  claim 221  wherein the first portion of plasma is platelet poor plasma and the second portion of plasma is platelet rich plasma.  
     
     
         225 . The method of  claim 221  wherein the first portion of plasma is platelet rich plasma and the second portion of plasma is platelet poor plasma.