Oncoprotein protein kinase

Abstract

An isolated polypeptide (JNK) characterized by having a molecular weight of 46 kD as determined by reducing SDS-PAGE, having serine and Threonine kinase activity, phosphorylating the c-Jun N-terminal activation domain and polynucleotide sequences and method of deterction of JNK are provided herein. JNK phosphorylates c-Jun N-terminal activation domain which affects gene expression from AP-1 sites.

1 . A method for identifying a composition which affects a kinase characterized as having serine and threonine kinase activity and phosphorylating a c-Jun N-terminal activation domain, comprising: 
 a) incubating components comprising the composition and the kinase or polynucleotide encoding the kinase, wherein the incubating is carried out under conditions sufficient to allow the components to interact; and    b) measuring the effect of the composition on the kinase or polynucleotide encoding the kinase.    
     
     
         2 - 9 . (canceled)