G protein-binding proteins and polynucleotides encoding the same

Abstract

Novel proteins which contain a structural module conserved in the G protein coupled receptor superfamily, polynucleotides which encode these proteins, and methods for producing these proteins are provided. Diagnostic, therapeutic, and screening methods employing the polynucleotides and polypeptides of the present invention are also provided.

1 . An isolated, recombinantly-produced or chemically-synthesized polypeptide comprising the amino acid sequence depicted in SEQ ID NO:6.  
     
     
         2 . A cell comprising the polypeptide of  claim 1 .  
     
     
         3 . An isolated, recombinantly-produced or chemically-synthesized polypeptide comprising the amino acid sequence depicted in SEQ ID NO:6 but with an arginine to glutamate substitution at residue 177.  
     
     
         4 . A cell comprising the polypeptide of  claim 3 .  
     
     
         5 . An isolated or recombinantly-produced protein comprising the amino acid sequence encoded by the cDNA insert of ATCC 98712 or ATCC 98852.  
     
     
         6 . A cell comprising the polypeptide of  claim 5 .  
     
     
         7 . An antibody immunoreactive to a polypeptide consisting of the amino acid sequence depicted in SEQ ID NO:6.  
     
     
         8 . An isolated, recombinantly-produced or chemically-synthesized splice variant of SEQ ID NO:6.  
     
     
         9 . An isolated, recombinantly-produced or chemically-synthesized polynucleotide comprising a nucleic acid sequence encoding the amino acid sequence depicted in SEQ ID NO:6.  
     
     
         10 . The polynucleotide of  claim 9 , wherein said nucleic acid sequence comprises nucleotides 6-668 of SEQ ID NO:5.  
     
     
         11 . The polynucleotide of  claim 9 , wherein said nucleic acid sequence comprises SEQ ID NO:5.  
     
     
         12 . The polynucleotide of  claim 9 , wherein said polynucleotide is an expression vector comprising an expression control sequence operably linked to said nucleic acid sequence.  
     
     
         13 . A cell comprising the polynucleotide of  claim 9 .  
     
     
         14 . An isolated, recombinantly-produced or chemically-synthesized polynucleotide comprising a nucleic acid sequence encoding the amino acid sequence depicted in SEQ ID NO:6 but with an arginine to glutamate substitution at residue 177.  
     
     
         15 . A cell comprising the polynucleotide of  claim 14 .  
     
     
         16 . An isolated or recombinantly-produced polynucleotide comprising the nucleic acid sequence of the cDNA insert of ATCC 98712 or ATCC 98852.  
     
     
         17 . A cell comprising the polynucleotide of  claim 16 .  
     
     
         18 . An isolated, recombinantly-produced or chemically-synthesized polynucleotide comprising a nucleic acid sequence selected from the group consisting of: 
 a splice variant of SEQ ID NO:5;    an allelic variant of SEQ ID NO:5; and    a nucleic acid sequence which is capable of hybridizing under (need the rejection) stringent conditions to SEQ ID NO:5, wherein the length of said nucleic acid sequence is at least 50% of that of SEQ ID NO:5.