Method for producing phospholipid

Abstract

A method for producing a phospholipid using transphosphatidylation, which comprises homogenizing a mixture of a raw material phospholipid, a hydroxyl-containing acceptor, phospholipase D, and water in the absence of an organic solvent to obtain a homogenized mixture; and subjecting the homogenized mixture to a transphosphatidylation reaction at 15° C. to 65° C. The homogenized mixture has a lamellar lyotropic liquid crystal structure. An objective phospholipid can be obtained from the homogenized mixture through transphophatidylation without using an organic solvent or calcium.

1 . A method for producing a phospholipid by transphosphatidylation comprising: 
 homogenizing a mixture of a raw material phospholipid, a hydroxyl-containing acceptor, phospholipase D and water in the absence of an organic solvent to obtain a homogenized mixture; and    subjecting said homogenized mixture to a transphosphatidylation reaction at a temperature within a range from 15° to 65° C.    
     
     
         2 . The method according to  claim 1 , wherein said homogenized mixture substantially has a lamellar lyotropic liquid crystal structure.  
     
     
         3 . The method according to  claim 1 , wherein the content of water in said homogenized mixture is adjusted within a range from 10 wt % to 100 wt % relative to the weight of the raw material phospholipid.  
     
     
         4 . The method according to  claim 1 , wherein the content of said hydroxyl-containing acceptor is adjusted within a range from 0.3 mole to 10 moles per 1 mole of the raw material phospholipid in said homogenization.  
     
     
         5 . The method according to  claim 1 , wherein said hydroxyl-containing acceptor is at least one hydroxyl-containing acceptor selected from the group consisting of serine, glycerol, L-ascorbic acid, glucose and choline.  
     
     
         6 . The method according to  claim 1 , wherein said hydroxyl-containing acceptor is serine, which yields a phosphatidylserine.  
     
     
         7 . The method according to  claim 1 , which further comprises adding an edible oil and/or a fat during said homogenization.  
     
     
         8 . The method according to  claim 6 , further comprising the steps of: 
 dissolving the phospholipid which contains said phosphatidylserine in an alcohol to obtain a solution; and    carrying out an insolubilization by adding a metallic salt to said solution to insolubilize the phosphatidylserine and separating the resultant insolubilized matter.    
     
     
         9 . The method according to  claim 8 , wherein said metallic salt is at least one metallic salt selected from the group comprising a lithium salt, a potassium salt, and a sodium salt.  
     
     
         10 . The method according to  claim 8 , wherein said metallic salt is lithium chloride, potassium chloride, or sodium chloride.  
     
     
         11 . The method according to  claim 8 , wherein the alcohol is ethyl alcohol.