Device and method for multiple analyte detection

Abstract

A method for making a test device is provided. The method can comprise providing (a) a substrate comprising a plurality of wells, wherein each well comprises at least one dimension no greater than 1 mm; (ii) a robotic dispensing system comprising at least one piezoelectric dispenser; and (iii) a liquid solution comprising at least one analyte-specific reagent. The method further comprises delivering a volume of the liquid solution into at least some of the wells using the at least one piezoelectric dispenser. The volume can be no greater than about 1 microliter. The method can further comprise placing a cover over the wells.

1 . A method for making a test device, comprising: 
 providing (a) a substrate comprising a plurality of wells, wherein each well comprises at least one dimension no greater than 1 mm; (ii) a robotic dispensing system comprising at least one piezoelectric dispenser; and (iii) a liquid solution comprising at least one analyte-specific reagent;    delivering a volume of said liquid solution into at least some of said wells, using said at least one piezoelectric dispenser, wherein said volume is no greater than about 1 microliter; and    placing a cover over said wells.    
     
     
         2 . The method of  claim 1 , further comprising, prior to said placing, at least partially drying said liquid solution.  
     
     
         3 . The method of  claim 2 , wherein said drying is effected, at least in part, by baking.  
     
     
         4 . The method of  claim 2 , wherein said drying is effected, at least in part, by reduced pressure.  
     
     
         5 . The method of  claim 1 , wherein said cover seals said wells.  
     
     
         6 . The method of  claim 1 , wherein said cover is optically transparent.  
     
     
         7 . The method of  claim 1 , wherein each of said wells comprises at least one dimension no greater than 0.2 mm.  
     
     
         8 . The method of  claim 1 , wherein said substrate comprises at least 49 wells within an area no greater than 3 cm×2 cm.  
     
     
         9 . The method of  claim 1 , wherein said at least one reagent comprises at least two oligonucleotides primers and a detectable probe.  
     
     
         10 . The method of  claim 1 , wherein said robotic dispensing system comprises a plurality of piezoelectric dispensers.