Nematode-extracted serine protease inhibitors and anticoagulant proteins

Abstract

Proteins which have activity as anticoagulants and/or serine protease inhibitors and have at least one NAP domain and are described. Certain of these proteins have factor Xa inhibitory activity and others have activity as inhibitors of factor VIIa/TF. These proteins can be isolated from natural sources as nematodes, chemically synthesized or made by recombinant methods using various DNA expression systems.

1 - 269 . (canceled)  
     
     
         270 . A pharmaceutical composition comprising: 
 (a) a polypeptide having the amino acid sequence LysAlaThrMetGlnCysGlyGluAsnGluLysTyrAspSerCysGlySerLysGluCysAspLys LysCysLysTyrAspGlyValGluGluGluAspAspGluGluProAsnValProCysLeuValArg ValCysHisGlnAspCysValCysGluGluGlyPheTyrArgAsnLysAspAspLysCysValSer AlaGluAspCysGluLeuAspAsnMetAspPheIleTyrProGlyThrArgAsnPro (rNAPc2/Pro); and    (b) a pharmaceutically acceptable carrier.    
     
     
         271 . A method for producing a polypeptide comprising the amino acid sequence of rNAPc2, the method comprising 
 (a) providing a cell encoding a polypeptide comprising the amino acid sequence of rNAPc2;    (b) culturing the cell under conditions suitable for the production of the polypeptide comprising the amino acid sequence of rNAPc2; and    (c) purifying the polypeptide from the cell culture supernatant.