US2005208481A1PendingUtilityA1
Diagnostic device using GP40
Individually held — no corporate assignee on recordPriority: Dec 5, 1988Filed: Nov 23, 2004Published: Sep 22, 2005
Est. expiryDec 5, 2008(expired)· nominal 20-yr term from priority
C07K 16/1145C07K 16/1143A61K 39/00G01N 33/56983C07K 14/005G01N 2469/20C12N 2740/14022G01N 33/6878G01N 2333/70503G01N 2333/15C12N 2740/15022
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Claims
Abstract
Assay methods and devices for detecting antibodies to Feline Immunedeficiency Virus (FIV) using an FIV envelope polypeptide such as recombinant gp40.
Claims
exact text as granted — not AI-modified1 - 13 . (canceled)
14 . A method for detecting antibody to FIV in a sample comprising:
providing an isolated Feline Immunodeficiency Virus (FIV) envelope polypeptide that reacts specifically with a monoclonal antibody that is specific for the FIV envelope protein gp130, said isolated polypeptide being the FIV transmembrane envelope protein (gp40) or a fragment thereof, and detecting a reaction between the polypeptide and antibody in the sample.
15 . The method of claim 14 in which the envelope polypeptide is gp40.
16 . The method of claim 15 in which the envelope polypeptide is recombinant gp40.
17 . The method of claim 15 in which the envelope polypeptide is synthesized by chemical methods.
18 . The method of any one of claims 14 - 17 in which the reagent comprises a binding moiety that binds to sample antibody reacted with the polypeptide.
19 . The method of claim 18 in which the binding moiety is attached to a color-producing moiety.
20 . An assay device for detecting antibody to FIV in a sample comprising:
an isolated Feline Immunodeficiency Virus (FIV) envelope polypeptide that reacts specifically with a monoclonal antibody that is specific for the FIV envelope protein gp130, said isolated polypeptide being the FIV transmembrane envelope protein (gp40) or a fragment thereof; and at least one reagent for detecting a reaction between the polypeptide and antibody in the sample.
21 . The device of claim 20 in which the envelope polypeptide is gp40.
22 . The device of claim 21 in which the envelope polypeptide is recombinant gp40.
23 . The device of claim 21 in which the envelope polypeptide is synthesized by chemical methods.
24 . The device of any one of claims 20 - 23 in which the reagent comprises a binding moiety that binds to sample antibody reacted with the polypeptide.
25 . The device of claim 20 in which the binding moiety is attached to a color-producing moiety.
26 . An ELISA assay device for detecting antibody to FIV in a sample comprising:
a capture protein consisting essentially of recombinant gp40; and at least one reagent for detecting a reaction between the capture protein and antibody in the sample.Join the waitlist — get patent alerts
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