US2005208481A1PendingUtilityA1

Diagnostic device using GP40

Individually held — no corporate assignee on recordPriority: Dec 5, 1988Filed: Nov 23, 2004Published: Sep 22, 2005
Est. expiryDec 5, 2008(expired)· nominal 20-yr term from priority
C07K 16/1145C07K 16/1143A61K 39/00G01N 33/56983C07K 14/005G01N 2469/20C12N 2740/14022G01N 33/6878G01N 2333/70503G01N 2333/15C12N 2740/15022
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Claims

Abstract

Assay methods and devices for detecting antibodies to Feline Immunedeficiency Virus (FIV) using an FIV envelope polypeptide such as recombinant gp40.

Claims

exact text as granted — not AI-modified
1 - 13 . (canceled)  
     
     
         14 . A method for detecting antibody to FIV in a sample comprising: 
 providing an isolated Feline Immunodeficiency Virus (FIV) envelope polypeptide that reacts specifically with a monoclonal antibody that is specific for the FIV envelope protein gp130, said isolated polypeptide being the FIV transmembrane envelope protein (gp40) or a fragment thereof, and    detecting a reaction between the polypeptide and antibody in the sample.    
     
     
         15 . The method of  claim 14  in which the envelope polypeptide is gp40.  
     
     
         16 . The method of  claim 15  in which the envelope polypeptide is recombinant gp40.  
     
     
         17 . The method of  claim 15  in which the envelope polypeptide is synthesized by chemical methods.  
     
     
         18 . The method of any one of claims  14 - 17  in which the reagent comprises a binding moiety that binds to sample antibody reacted with the polypeptide.  
     
     
         19 . The method of  claim 18  in which the binding moiety is attached to a color-producing moiety.  
     
     
         20 . An assay device for detecting antibody to FIV in a sample comprising: 
 an isolated Feline Immunodeficiency Virus (FIV) envelope polypeptide that reacts specifically with a monoclonal antibody that is specific for the FIV envelope protein gp130, said isolated polypeptide being the FIV transmembrane envelope protein (gp40) or a fragment thereof; and    at least one reagent for detecting a reaction between the polypeptide and antibody in the sample.    
     
     
         21 . The device of  claim 20  in which the envelope polypeptide is gp40.  
     
     
         22 . The device of  claim 21  in which the envelope polypeptide is recombinant gp40.  
     
     
         23 . The device of  claim 21  in which the envelope polypeptide is synthesized by chemical methods.  
     
     
         24 . The device of any one of claims  20 - 23  in which the reagent comprises a binding moiety that binds to sample antibody reacted with the polypeptide.  
     
     
         25 . The device of  claim 20  in which the binding moiety is attached to a color-producing moiety.  
     
     
         26 . An ELISA assay device for detecting antibody to FIV in a sample comprising: 
 a capture protein consisting essentially of recombinant gp40; and    at least one reagent for detecting a reaction between the capture protein and antibody in the sample.

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