Novel protein and method for producing the protein

Abstract

A novel protein which binds to Osteoclastogenesis Inhibitory Factor (OCIF-binding molecule; OBM), a process for preparing the same, DNA encoding said protein, a protein having an amino acid sequence encoded by this DNA, a method for producing said protein by genetic engineering technique, and a pharmaceutical composition containing said protein. Screening methods for a substance for controlling expression of said protein using said protein and the DNA, a substance which inhibits or modulates the biological activity of said protein, or a receptor which transmits the action of said protein through binding to said protein, the substance obtained by the screening methods, and a pharmaceutical composition which contains this substance. An antibody for said protein, a process for preparing the same, a measuring method of said protein using the antibody, and a medicine comprising this antibody.

1 - 64 . (canceled)  
     
     
         65 . An isolated antibody that binds with a higher dissociation constant to a murine OBM polypeptide than to a human OBM polypeptide.  
     
     
         66 . An isolated antibody that binds with a higher dissociation constant to a murine OBM polypeptide as shown in SEQ ID NO: 16 than to a human OBM polypeptide as shown in SEQ ID NO: 11.  
     
     
         67 . An isolated antibody that binds with a higher dissociation constant to a murine OBM polypeptide as shown in SEQ ID NO: 1 than to a human OBM polypeptide as shown in SEQ ID NO: 11.  
     
     
         68 . An antibody according to  claim 65  which is a monoclonal antibody.  
     
     
         69 . A method of producing a monoclonal antibody according to  claim 68 , said method comprising culturing a cloned hybridoma cell that produces said antibody.  
     
     
         70 . A method of producing a monoclonal antibody according to  claim 68 , said method comprising injecting into the peritoneal cavity of a rodent a cloned hybridoma cell that produces said antibody.  
     
     
         71 . A cloned hybridoma cell that produces a monoclonal antibody according to  claim 68 .  
     
     
         72 . A composition comprising an antibody according to  claim 65 .  
     
     
         73 . A purified antibody that binds with higher dissociation constant to a murine RANKL polypeptide than to a human RANKL polypeptide, wherein said antibody is generated by a method comprising immunizing with a RANKL polypeptide comprising amino acids 73-317 of SEQ ID NO: 11.  
     
     
         74 . A purified antibody that binds with higher dissociation constant to a murine RANKL polypeptide as shown in SEQ ID NO: 16 than to a human RANKL polypeptide, wherein said antibody is generated by a method comprising immunizing with a RANKL polypeptide comprising amino acids 73-317 of SEQ ID NO: 11.  
     
     
         75 . A purified antibody that binds with higher dissociation constant to a murine RANKL polypeptide as shown in SEQ ID NO: 1 than to a human RANKL polypeptide, wherein said antibody is generated by a method comprising immunizing with a RANKL polypeptide comprising amino acids 73-317 of SEQ ID NO: 11.  
     
     
         76 . An antibody according to  claim 73  which is a monoclonal antibody.  
     
     
         77 . A composition comprising an antibody according to  claim 76 .  
     
     
         78 . A purified antibody that binds to a human RANKL polypeptide, but that does not bind to a murine RANKL polypeptide.  
     
     
         79 . A purified antibody that binds to a human RANKL polypeptide as shown in SEQ ID NO: 11, but that does not bind to a murine RANKL polypeptide as shown in SEQ ID NO: 1.  
     
     
         80 . A purified antibody that binds to a human RANKL polypeptide as shown in SEQ ID NO: 11, but that does not bind to a murine RANKL polypeptide as shown in SEQ ID NO: 16.  
     
     
         81 . A purified antibody that binds with higher dissociation constant to a murine OBM polypeptide than to a human OBM polypeptide, wherein said antibody is generated by a method comprising immunizing with a human RANKL polypeptide.  
     
     
         82 . A purified antibody that binds with higher dissociation constant to a murine OBM polypeptide as shown in SEQ ID NO: 16 than to a human OBM polypeptide as shown in SEQ ID NO: 11, wherein said antibody is generated by a method comprising immunizing with a RANKL polypeptide comprising amino acids 73-317 of SEQ ID NO: 11.  
     
     
         83 . A purified antibody that binds with higher dissociation constant to a murine OBM polypeptide as shown in SEQ ID NO: 1 than to a human OBM polypeptide as shown in SEQ ID NO: 11, wherein said antibody is generated by a method comprising immunizing with a RANKL polypeptide comprising amino acids 73-317 of SEQ ID NO: 11.  
     
     
         84 . A method for generating an antibody, said method comprising immunizing with a RANKL polypeptide selected from the group consisting of a polypeptide comprising amino acids 1-317 of SEQ ID NO: 1I1 and a polypeptide comprising amino acids 73-317 of SEQ ID NO: 11.  
     
     
         85 . A method for generating an antibody, said method comprising immunizing with a RANKL polypeptide selected from the group consisting of a polypeptide comprising amino acids 1-317 of SEQ ID NO: 11.