Arcelin-5 promoter and uses thereof

Abstract

The present invention discloses Arcelin promoters capable of transcribing a heterologous nucleic acid sequence at high levels in plants. The promoters are particularly suited for use in soybean plants and plant cells. Methods of modifying, producing, and using the promoters are also disclosed. The invention further discloses compositions, transformed host cells, transgenic plants, and seeds containing the high-expression Arcelin promoters, and methods for preparing and using the same.

1 . A transformed soybean plant cell containing a nucleic acid molecule that comprises in the 5′ to 3′ direction: 
 a promoter having a nucleic acid sequence of SEQ ID NO: 2;    operably linked to a structural nucleic acid sequence;    wherein the promoter is heterologous with respect to the structural nucleic acid sequence.    
     
     
         2 - 4 . (canceled)  
     
     
         5 . The transformed soybean plant cell of  claim 1 , wherein the structural nucleic acid sequence encodes a protein selected from the group consisting of gamma methyltransferase, phytyl prenyltransferase, beta-ketoacyl-CoA synthase, fatty acyl-CoA reductase, fatty acyl CoA:fatty alcohol transacylase, anthranilate synthase, threonine deaminase, acetohydroxy acid synthase, aspartate kinase, dihydroxy acid synthase, aspartate kinase, dihydropicolinate synthase, thioesterase, 7Sα′ seed storage protein, 11S seed storage protein, glycinin, beta-conglycinin, phaseolin, maize globulin-1, maize zeins, seed albumin, and seed lectin.  
     
     
         6 . The transformed soybean plant cell of  claim 1 , wherein the nucleic acid molecule further comprises a 5′ leader sequence.  
     
     
         7 . The transformed soybean plant cell of  claim 6 , wherein the 5′ leader sequence is selected from the group consisting of Arcelin-5 5′, dSSU 5′, PetHSP70 5′, and GmHSP17.9 5′.  
     
     
         8 . The transformed soybean plant cell of  claim 1 , wherein the nucleic acid molecule further comprises a 3′untranslated region.  
     
     
         9 . The transformed soybean plant cell of  claim 8 , wherein the 3′untranslated region is selected from the group consisting of Arcelin-5 3′, NOS 3′, E9 3′, ADR12 3′, 7Sα′ 3′, 11S 3′, and albumin 3′.  
     
     
         10 . The transformed soybean plant cell of  claim 1 , wherein the promoter expresses the structural nucleic acid sequence in an amount greater than 2.5% (w/w) of the total cellular RNA or protein.  
     
     
         11 . The transformed soybean plant cell of  claim 10 , wherein the promoter expresses the structural nucleic acid sequence in an amount greater than 5% (w/w) of the total cellular RNA or protein.  
     
     
         12 . The transformed soybean plant cell of  claim 11 , wherein the promoter expresses the structural nucleic acid sequence in an amount greater than 10% (w/w) of the total cellular RNA or protein.  
     
     
         13 . A transgenic soybean plant containing a nucleic acid molecule that comprises in the 5′ to 3′ direction: 
 a promoter having a nucleic acid sequence of SEQ ID NO: 2;    operably linked to a structural nucleic acid sequence;    wherein the promoter is heterologous with respect to the structural nucleic acid sequence.    
     
     
         14 - 15 . (canceled)  
     
     
         16 . The transgenic soybean plant of  claim 13 , wherein the promoter is SEQ ID NO: 2.  
     
     
         17 . The transgenic soybean plant of  claim 13 , wherein the structural nucleic acid sequence encodes a protein selected from the group consisting of gamma methyltransferase, phytyl prenyltransferase, beta-ketoacyl-CoA synthase, fatty acyl-CoA reductase, fatty acyl CoA:fatty alcohol transacylase, anthranilate synthase, threonine deaminase, acetohydroxy acid synthase, aspartate kinase, dihydroxy acid synthase, aspartate kinase, dihydropicolinate synthase, thioesterase, 7Sα′ seed storage protein, 11 S seed storage protein, glycinin, beta-conglycinin, phaseolin, maize globulin-1, maize zeins, seed albumin, and seed lectin.  
     
     
         18 . The transgenic soybean plant of  claim 13 , wherein the nucleic acid molecule further comprises a 5′ leader sequence.  
     
     
         19 . The transgenic soybean plant of  claim 18 , wherein the 5′ leader sequence is selected from the group consisting of Arcelin-5 5′, dSSU 5′, PetHSP70 5′, and GmHSP17.9 5′.  
     
     
         20 . The transgenic soybean plant of  claim 13 , wherein the nucleic acid molecule further comprises a 3′ untranslated region.  
     
     
         21 . The transgenic soybean plant of  claim 20 , wherein the 3′ untranslated region is selected from the group consisting of Arcelin-5 3′, NOS 3′, E9 3′, ADR12 3′, 7Sα′ 3′, 11S 3′, and albumin 3′.  
     
     
         22 . The transgenic soybean plant of  claim 13 , wherein the promoter expresses the structural nucleic acid sequence in an amount greater than 2.5% (w/w) of the total cellular RNA or protein.  
     
     
         23 . The transgenic soybean plant of  claim 22 , wherein the promoter expresses the structural nucleic acid sequence in an amount greater than 5% (w/w) of the total cellular RNA or protein.  
     
     
         24 . The transgenic soybean plant of  claim 23 , wherein the promoter expresses the structural nucleic acid sequence in an amount greater than 10% (w/w) of the total cellular RNA or protein.