Low temperature responsive nucleotide sequences and uses thereof

Abstract

This disclosure provides gene sequence of a novel gene DaRub1 of Deschampsia antarctica expressing in low temperatures. The promoter sequence of this gene is identified and characterized. The promoter is inducible by low temperatures, wounding and auxin treatment. Additionally the disclosure shows improved low temperature tolerance of transgenic Eucalyptus plants expressing DaRub1 gene.

1 . A nucleotide sequence encoding for amino acid sequence essentially according to SEQ ID NO:4; said nucleotide sequence having coding sequence according to SEQ ID NO:3.  
     
     
         2 . The nucleotide sequence according to  claim 1 , wherein the nucleotide sequence further comprises promoter sequence according to SEQ ID NO:2.  
     
     
         3 . The nucleotide sequence according to  claim 1 , wherein the nucleotide sequence is essentially according to SEQ ID NO: 1.  
     
     
         4 . An inducible promoter having nucleotide sequence according to SEQ ID NO:2.  
     
     
         5 . The promoter according to  claim 4 , wherein the promoter is inducible by low temperature, wounding and auxin.  
     
     
         6 . A method for conferring low temperature resistance in a plant, said method comprising a step of introducing into the plant a recombinant expression construct, said construct further comprising a plant promoter operably linked to DaRUB1 sequence essentially according to SEQ ID NO:3.  
     
     
         7 . The method according to  claim 6 , wherein the promoter is essentially according to SEQ ID NO:2.  
     
     
         8 . The method according to  claim 6 , wherein the sequence encodes a gene product having a amino acid sequence essentially according to SEQ ID NO:4.  
     
     
         9 . The method according to  claim 6 , wherein the plant is  Euclayptus globulus.    
     
     
         10 . The method according to  claim 6 , wherein the plant is  Arabidopsis thaliana.    
     
     
         11 . The method according to  claim 6 , wherein the plant species is a fruit tree.  
     
     
         12 . The method according to  claim 11 , wherein the plant species is selected from the group consisting of peach, avocado and orange.  
     
     
         13 . A DNA construct for cloning and transforming plants, said construct comprising nucleotide sequence selected from a group consisting nucleotide sequences according to SEQ ID NO:2 and SEQ ID NO:3.  
     
     
         14 . A transgenic plant comprising the DNA construct according to  claim 13 .  
     
     
         15 . The transgenic plant according to  claim 14 , wherein the plant comprises DNA construct according to SEQ ID NO:3 and the plant further has an increased low temperature tolerance.  
     
     
         16 . The transgenic plant according to  claim 15 , wherein the plant is an  Eucalyptus globulus  plant.  
     
     
         17 . The transgenic plant according to  claim 15 , wherein the plant is an  Arabidobsis thalina  plant.  
     
     
         18 . The transgenic plant according to  claim 15 , wherein the plant is a fruit tree.  
     
     
         19 . The transgenic plant according to  claim 18  wherein the plant is selected from the group consisting of peach, avocado and orange.