Antisense modulation of p70 S6 kinase expression

Abstract

Antisense compounds, compositions and methods are provided for modulating the expression of p70 S6 kinase. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding p70 S6 kinase. Methods of using these compounds for modulation of p70 S6 kinase expression and for treatment of diseases associated with expression of p70 S6 kinase are provided.

1 . A compound 8 to 50 nucleobases in length targeted to a nucleic acid molecule encoding p70 S6 kinase, wherein said compound specifically hybridizes with and inhibits the expression of p70 S6 kinase.  
     
     
         2 . The compound of  claim 1  which is an antisense oligonucleotide.  
     
     
         3 . The compound of  claim 2  wherein the antisense oligonucleotide has a sequence comprising SEQ ID NO: 10, 11, 12, 13, 14, 16, 18, 19, 20, 21, 22, 23, 24, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 46, 47, 48 or 49.  
     
     
         4 . The compound of  claim 2  wherein the antisense oligonucleotide has a sequence comprising SEQ ID NO: 11, 12, 13, 14, 16, 20, 21, 22, 23, 29, 30, 31, 34, 37, 38, 41, 43, 44, 46 or 48.  
     
     
         5 . The compound of  claim 2  wherein the antisense oligonucleotide comprises at least one modified internucleoside linkage.  
     
     
         6 . The compound of  claim 5  wherein the modified internucleoside linkage is a phosphorothioate linkage.  
     
     
         7 . The compound of  claim 2  wherein the antisense oligonucleotide comprises at least one modified sugar moiety.  
     
     
         8 . The compound of  claim 7  wherein the modified sugar moiety is a 2′-O-methoxyethyl sugar moiety.  
     
     
         9 . The compound of  claim 2  wherein the antisense oligonucleotide comprises at least one modified nucleobase.  
     
     
         10 . The compound of  claim 9  wherein the modified nucleobase is a 5-methylcytosine.  
     
     
         11 . The compound of  claim 2  wherein the antisense oligonucleotide is a chimeric oligonucleotide.  
     
     
         12 . A compound 8 to 50 nucleobases in length which specifically hybridizes with at least an 8-nucleobase portion of an active site on a nucleic acid molecule encoding p70 S6 kinase.  
     
     
         13 . A composition comprising the compound of  claim 1  and a pharmaceutically acceptable carrier or diluent.  
     
     
         14 . The composition of  claim 13  further comprising a colloidal dispersion system.  
     
     
         15 . The composition of  claim 13  wherein the compound is an antisense oligonucleotide.  
     
     
         16 . A method of inhibiting the expression of p70 S6 kinase in cells or tissues comprising contacting said cells or tissues with the compound of  claim 1  so that expression of p70 S6 kinase is inhibited.  
     
     
         17 . A method of treating an animal having a disease or condition associated with p70 S6 kinase comprising administering to said animal a therapeutically or prophylactically effective amount of the compound of  claim 1  so that expression of p70 S6 kinase is inhibited.  
     
     
         18 . The method of  claim 17  wherein the disease or condition is a hyperproliferative disorder.  
     
     
         19 . The method of  claim 18  wherein the hyperproliferative disorder is cancer.  
     
     
         20 . The method of  claim 16  wherein the disease or condition is a metabolic disorder.