US2005278802A1PendingUtilityA1

Methods for tissue culturing and transforming elite inbreds of Zea mays L.

Assignee: WILSON HERBERT MPriority: Dec 1, 1998Filed: Jul 18, 2005Published: Dec 15, 2005

Est. expiryDec 1, 2018(expired)· nominal 20-yr term from priority

Inventors:Herbert Martin Wilson Bruce Held

C12N 15/8205A01H 4/008

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Abstract

The present invention is directed to methods for the tissue culture and transformation of elite inbreds of corn ( Zea mays L.). More specifically, the present invention is directed to a method for initiating Type II callus from corn tissue. The present invention is also directed to a method for enhancing the integration of foreign DNA in the transformation of corn using a heat shock treatment. The present invention is further directed to a method of transforming elite inbreds of corn using Agrobacterium.

Claims

exact text as granted — not AI-modified

1 - 2 . (canceled)  
   
   
       3 . The method of  claim 35 , wherein said monosaccharide sugar is glucose.  
   
   
       4 - 34 . (canceled)  
   
   
       35 . A method for producing a corn plant comprising the steps of: 
 (a) culturing a corn embryo on a medium comprising a compound selected from the group consisting of glucose, maltose, lactose, sorbitol, and mannitol, wherein said compound is in an amount of from about 5 g/L to about 30 g/L, to produce a type II callus; and    (b) regenerating a plant.

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