Light scanning microscope and use

Abstract

Light scanning microscope with an at least single-dimensional light distribution for grid-shaped illumination of a sample in a locally limited grid field and detector means for recording sample light as well as a sample table that moves in at least one direction, whereby in a first process step an illumination of the sample, detection of sample light and data recording of the detection is carried out during a movement of the sample table in at least a first direction over the dimensions of the grid field and the recording of the respective table position is assigned to the data recording.

1 - 5 . (canceled)  
     
     
         6 . Laser scanning microscope comprising: 
 a sample table movable in at least one direction over the dimensions of a grid field,    means for creating an at least one-dimensional light distribution for grid-shaped illumination of a sample in a locally limited grid field, and for illuminating the sample during movement of the sample table in at least a first direction,    detector means for detecting and recording sample light during movement of the sample table in the at least one first direction and for recording of the position of the sample table.    
     
     
         7 . Light scanning microscope according to  claim 6 , wherein the grid field is generated by movement of the light distribution in at least one direction.  
     
     
         8 . Light scanning microscope according to  claim 6 , wherein the sample table is movable in a second direction perpendicular to the first direction.  
     
     
         9 . Light scanning microscope according to  claim 6 , wherein several scanning regions are adjacent to each other and wherein the microscope further comprises means for composing an image from the stored scanning regions.  
     
     
         10 . Light scanning microscope according to  claim 9 , wherein the scanning regions overlap and wherein the microscope further comprises means for correcting the stored table position for at least one sample region using image comparisons.  
     
     
         11 . Method of using the laser scanning microscope of  claim 6 , comprising the steps of: 
 (a) carrying out illumination of the sample, detection of sample light and data recording of the detection during movement of the sample table in at least one first direction over the dimensions of the grid field, and    (b) recording of the respective position of the sample table using the detector means.    
     
     
         12 . Method according to  claim 11 , further comprising the step of generating the grid field by movement of the light distribution in at least one direction.  
     
     
         13 . Method according to  claim 11 , further comprising the steps of: 
 (c) moving the sample table in a direction perpendicular to the first direction occurs,    (d) storing the movement position is stored, and    (e) repeating steps (a) and (b) for imaging several scanning regions.    
     
     
         14 . Method according to  claim 11 , wherein several scanning regions are adjacent to each other, the method further comprising the step of composing an image from the stored scanning regions.  
     
     
         15 . Method according to  claim 14 , wherein the scanning regions overlap, the method further comprising the step of correcting the stored table position for at least one sample region using image comparisons.