Method for diagnosing cancer by detecting gpc3

Abstract

Provided is a method for diagnosing cancer by detecting a novel cancer marker. Cancer can be diagnosed by detecting soluble glypican 3 in a test sample.

1 . A method for diagnosing cancer, which comprises detecting a soluble GPC3 protein in a test sample.  
     
     
         2 . The method for diagnosing cancer of  claim 1 , wherein the soluble GPC3 protein is a N-terminal peptide of GPC3.  
     
     
         3 . The method for diagnosing cancer of  claim 2 , wherein the N-terminal peptide of GPC3 is a peptide fragment contained in an amino acid sequence of GPC3 consisting of the 1 st amino acid to the 374th amino acid, or an amino acid sequence of GPC3 consisting of the 1st amino acid to the 358th amino acid.  
     
     
         4 . The diagnosis method of  claim 1 , wherein the test sample is selected from the group consisting of blood, serum and plasma.  
     
     
         5 . The diagnosis method of  claim 1 , wherein the cancer is hepatic cancer.  
     
     
         6 . The method of  claim 1 , comprising using an anti-GPC3 antibody.  
     
     
         7 . The method of  claim 6 , comprising using an anti-GPC3 antibody immobilized on a carrier and an anti-GPC3 antibody labeled with a labeling substances.  
     
     
         8 . The method of  claim 7 , wherein the labeling substances are biotin.  
     
     
         9 . A diagnostic reagent for cancer, comprising an anti-GPC3 antibody.  
     
     
         10 . The diagnostic reagent of  claim 9 , comprising an anti-GPC3 antibody immobilized on a carrier and an antibody labeled with a labeling substance.  
     
     
         11 . The diagnostic reagent of  claim 9 , wherein the cancer is hepatic cancer.  
     
     
         12 . The diagnostic reagent of  claim 9 , wherein the anti-GPC3 antibody recognizes the N-terminal peptide of GPC3.  
     
     
         13 . A diagnostic kit, comprising an anti-GPC3 antibody.  
     
     
         14 . The diagnostic kit of  claim 13 , comprising the anti-GPC3 antibody immobilized on carriers, and an antibody labeled with a labeling substance.  
     
     
         15 . The diagnosis method of  claim 3 , wherein the test sample is selected from the group consisting of blood, serum and plasma.  
     
     
         16 . The diagnosis method of  claim 3 , wherein the cancer is hepatic cancer.  
     
     
         17 . The diagnosis method of  claim 4 , wherein the cancer is hepatic cancer.  
     
     
         18 . The method of  claim 3 , comprising using an anti-GPC3 antibody.  
     
     
         19 . The diagnostic reagent of  claim 10 , wherein the cancer is hepatic cancer.  
     
     
         20 . The diagnostic reagent of any one of  claim 11 , wherein the anti-GPC3 antibody recognizes the N-terminal peptide of GPC3.