Biological fertilizer compositions comprising swine manure

Abstract

The present invention provides biological fertilizer compositions that comprise yeast cells and swine manure. The yeast cells of the invention have an enhanced ability to fix atmospheric nitrogen, decompose phosphorus minerals and compounds, decompose potassium minerals and compounds, decompose complex carbon compounds, overproduce growth factors, overproduce ATP, decompose undesirable chemicals, suppress growth of pathogenic microorganisms, or reduce undesirable odor. The biological fertilizer composition of the invention can replace mineral fertilizers in supplying nitrogen, phosphorus, and potassium to crop plants. Methods of manufacturing biological fertilizer compositions, and methods of uses are also encompassed.

1 - 2 . (canceled)  
   
   
       3 . A biological fertilizer composition comprising: 
 (I) swine manure;    (II) at least one of the following: 
 (a) a first yeast cell component prepared by culturing a first plurality of yeast cells in a first electromagnetic field having a frequency in the range of 840 to 916 MHz and a field strength of 10 to 200 mV/cm;  
 (b) a second yeast cell component prepared by culturing a second plurality of yeast cells in a second electromagnetic field having a frequency in the range of 300 to 500 MHz and a field strength of 10 to 300 mV/cm;  
 (c) a third yeast cell component prepared by culturing a third plurality of yeast cells in a third electromagnetic field having a frequency in the range of 190 to 285 MHz and a field strength of 10 to 200 mV/cm; and  
   (III) at least one of the following: 
 (d) a fourth yeast cell component prepared by culturing a fourth plurality of yeast cells in a fourth electromagnetic field having a frequency in the range of 30 to 50 MHz and a field strength of 20 to 200 mV/cm;  
 (e) a fifth yeast cell component prepared by culturing a fifth plurality of yeast cells in a fifth electromagnetic field having a frequency in the range of 70 to 100 MHz and a field strength in the range of 40 to 250 mV/cm; and  
 (f) a sixth yeast cell component prepared by culturing a sixth plurality of yeast cells in a sixth electromagnetic field having a frequency in the range of 2160 to 2250 MHz and 2280 to 2380 MHz and a field strength in the range of 100 to 300 mV/cm.  
   
   
   
       4 . The biological fertilizer composition of  claim 3 , further comprising at least one of the following: 
 (g) a seventh yeast cell component prepared by culturing a seventh plurality of yeast cells in a fourth electromagnetic field having a frequency in the range of to 1050 to 1160 MHz and a field strength of 10 to 200 mV/cm;    (h) an eighth yeast cell component prepared by culturing an eighth plurality of yeast cells in an eighth electromagnetic field having a frequency in the range of 1340 to 1440 MHz and a field strength of 20 to 200 mV/cm; and    (i) a ninth yeast cell component prepared by culturing a ninth plurality of yeast cells in a ninth electromagnetic field having a frequency in the range of 1630 to 1730 MHz and a field strength of 20 to 200 mV/cm.    
   
   
       5 - 15 . (canceled)  
   
   
       16 . A method of producing the biological fertilizer composition of  claim 3  or  4 , comprising in the order stated: 
 (I) preparing a mixture of yeast cells by mixing at least one yeast cell component (a), (b), or (c); with at least one yeast cell component (d), (e), or (f); and    (II) adding swine manure to said mixture of yeast cells to form the biological fertilizer composition.    
   
   
       17 . (canceled)  
   
   
       18 . The method of  claim 16 , wherein: 
 the first yeast cell component of (a) is prepared by culturing a first plurality of yeast cells in a first electromagnetic field or a first series of electromagnetic fields having a frequency in the range of 840 to 916 MHz and a field strength of 10 to 200 mV/cm;    the second yeast cell component of (b) prepared by culturing a second plurality of yeast cells in a second electromagnetic field or a second series of electromagnetic fields having a frequency in the range of 380 to 485 MHz and a field strength of 10 to 300 mV/cm;    the third yeast cell component of (c) is prepared by culturing a third plurality of yeast cells in a third electromagnetic field or a third series of electromagnetic fields having a frequency in the range of 190 to 285 MHz and a field strength of 10 to 200 mV/cm;    the fourth yeast cell component of (d) is prepared by culturing a fourth plurality of yeast cells in a fourth electromagnetic field or a fourth series of electromagnetic fields having a frequency in the range of 30 to 50 MHz and a field strength of 20 to 200 mV/cm;    the fifth yeast cell component of (e) is prepared by culturing a fifth plurality of yeast cells in a fifth electromagnetic field or a fifth series of electromagnetic fields having a frequency in the range of 70 to 100 MHz and a field strength in the range of 40 to 250 mV/cm; and    the sixth yeast cell component of (f) is prepared by culturing a sixth plurality of yeast cells in a sixth electromagnetic field or a sixth series of electromagnetic fields having a frequency in the range of 2160 to 2250 MHz and 2280 to 2380 MHz and a field strength in the range of 100 to 300 mV/cm.    
   
   
       19 . The method of  claim 18 , wherein: 
 the seventh yeast cell component of (g) is prepared by culturing a seventh plurality of yeast cells in a seventh electromagnetic field or a seventh series of electromagnetic fields having a frequency in the range of 1050 to 1160 MHz and a field strength of 10 to 200 mV/cm;    the eighth yeast cell component of (h) is prepared by culturing an eighth plurality of yeast cells in an eighth electromagnetic field or a eighth series of electromagnetic fields having a frequency in the range of 1340 to 1440 MHz and a field strength of 20 to 200 mV/cm; and    the ninth yeast cell component of (i) is prepared by culturing a ninth plurality of yeast cells in a ninth electromagnetic field or a ninth series of electromagnetic fields having a frequency in the range of 1630 to 1730 MHz and a field strength of 20 to 200 mV/cm.    
   
   
       20 - 23 . (canceled)  
   
   
       24 . A method for enhancing plant growth comprising growing the plant in the presence of a biological fertilizer composition of  claim 3  or  4 .  
   
   
       25 . The method of  claim 24  wherein the biological fertilizer composition is applied to soil at the depth of the major root system of the plant.  
   
   
       26 . The method of  claim 24  wherein about 600 to 1350 kg/ha of the biological fertilizer composition is used.  
   
   
       27 . The method of  claim 24  wherein the plant is a cereal crop, vegetable crop, fruit crop, flower crop, or grass crops.  
   
   
       28 . The method of  claim 24  wherein the plant is wheat, barley, corn, soybean, rice, oat, potato, apple, orange, tomato, melon, cherry, lemon, lettuce, carrot, sugar cane, tobacco, or cotton.  
   
   
       29 . The biological fertilizer composition of  claim 3  or  4  wherein the yeast cell component (b) is prepared by culturing a second plurality of yeast cells in a second electromagnetic field having a frequency in the range of 380 to 485 MHz and a field strength of 90 to 300 mV/cm.