Human cervical cancer protooncogene and protein encoded therein

Abstract

A human cervical cancer 1 protooncogene having a base sequence of SEQ ID:1 or a fragment thereof is overexpressed in various cancer tissues and can be used in diagnosing various cancers and an anti-sense gene complementary thereto can be used in treating cancers.

1 . A human cervical cancer 1 protooncogene having the base sequence of SEQ ID NO:1 or a fragment thereof.  
     
     
         2 . The fragment of the protooncogene of  claim 1  having a base sequence corresponding to base Nos. 9 to 1088 of SEQ ID NO:1.  
     
     
         3 . A protein having the amino acid sequence of SEQ ID:2 or a fragment thereof.  
     
     
         4 . A vector comprising the protooncogene or fragment of  claim 1 .  
     
     
         5 . A microorganism transformed with the vector of  claim 4 .  
     
     
         6 . The microorganism of  claim 5 , which is  E. coli  JM109/HCCR-1 (Accession No.: KCTC 0667BP).  
     
     
         7 . A process for preparing the protein or fragment of  claim 3  comprising culturing the microorganism of  claim 5  or  6 .  
     
     
         8 . A kit for diagnosis of cancer which comprises the protooncogene or fragment of  claim 1  or  2 .  
     
     
         9 . A kit for diagnosis of cancer which comprises the protein or fragment of  claim 3 .  
     
     
         10 . An anti-sense gene having a base sequence which is complementary to the sequence of the full or partial mRNA transcribed from the protooncogene or fragment of  claim 1  or  2  and being capable of binding the mRNA to inhibit the expression of said protooncogene or fragment.  
     
     
         11 . The anti-sense gene of  claim 10  having the base sequence of SEQ ID NO:3.  
     
     
         12 . A process for treating or preventing cancer in human which comprises administering a therapeutically effective amount of the anti-sense gene of  claim 10  or  11  to the human.