US2006029945A1PendingUtilityA1
Novel full length cDNA
Est. expiryNov 5, 2021(expired)· nominal 20-yr term from priority
Inventors:Takao IsogaiTomoyasu SugiyamaTetsuji OtsukiAi WakamatsuHiroyuki SatoShizuko IshiiJun-Ichi YamamotoYuuko IsonoYuri HioKaoru OtsukaKeiichi NagaiRyotaro IrieIchiro TamechikaNaohiko SekiTsutomu YoshikawaMotoyuki OtsukaKenji NagahariYasuhiko Masuho
C07K 14/47
48
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Claims
Abstract
Novel full-length cDNAs are provided. 1970 cDNA derived from human have been isolated. The full-length nucleotide sequences of the cDNA and amino acid sequences encoded by the nucleotide sequences have been determined. Because the cDNA of the present invention are full-length and contain the translation start site, they provide information useful for analyzing the functions of the polypeptide.
Claims
exact text as granted — not AI-modified1 . (canceled)
2 . A polypeptide encoded by a polynucleotide selected from the group consisting of the following (a) to (g):
(a) a polynucleotide comprising a protein-coding region of the nucleotide sequence of any one of SEQ ID NOs: 1 to 1970: (b) a polynucleotide encoding a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 1971 to 3940: (c) a polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 1971 to 3940, wherein in said amino acid sequence, one or more amino acids have been substituted, deleted, inserted and/or added, and wherein said nucleotide sequence encodes a polypeptide functionally equivalent to a polypeptide comprising the selected amino acid sequence; (d) a polynucleotide hybridizing to a polynucleotide comprising the nucleotide sequence of any one of SEQ ID NOs: 1 to 1970, wherein said nucleotide sequence encodes a polypeptide functionally equivalent to a polypeptide encoded by the selected nucleotide sequence; (e) a polynucleotide comprising a nucleotide sequence encoding a partial amino acid sequence of a polypeptide encoded by the polynucleotide according to any one of (a) to (d); (f) a polynucleotide comprising a nucleotide sequence having at least 70% identity to the nucleotide sequence of any one of SEQ ID NOs: 1 to 1970; and (g) a polynucleotide comprising a nucleotide sequence having at least 90% identity to the nucleotide sequence of any one of SEQ ID NOs: 1 to 1970, or a partial peptide thereof.
3 - 7 . (canceled)
8 . A method for producing the polypeptide or the partial peptide of claim 2 , said method comprising the steps of culturing a transformant carrying a polynucleotide selected from the group consisting of the following (a) to (g):
(a) a polynucleotide comprising a protein-coding region of the nucleotide sequence of any one of SEQ ID NOs: 1 to 1970; (b) a polynucleotide encoding a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 1971 to 3940; (c) a polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 1971 to 3940, wherein, in said amino acid sequence, one or more amino acids have been substituted, deleted, inserted and/or added, and wherein said nucleotide sequence encodes a polypeptide functionally equivalent to a polypeptide comprising the selected amino acid sequence; (d) a polynucleotide hybridizing to a polynucleotide comprising the nucleotide sequence of any one of SEQ ID NOs: 1 to 1970, wherein said nucleotide sequence encodes a polypeptide functionally equivalent to a polypeptide encoded by the selected nucleotide sequence; (e) a polynucleotide comprising a nucleotide sequence encoding a partial amino acid sequence of a polypeptide encoded by the polynucleotide according to any one of (a) to (d); (f) a polynucleotide comprising a nucleotide sequence having at least 70% identity to the nucleotide sequence of any one of SEQ ID NOs: 1 to 1970; and (g) a polynucleotide comprising a nucleotide sequence having at least 90% identity to the nucleotide sequence of any one of SEQ ID NOs: 1 to 1970, and recovering an expression product.
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