Polynucleotides encoding polypeptides involved in the stress response to environmental changes in Methylophilus methylotrophus

Abstract

The present invention provides polypeptides and polynucleotides involved in the stress response to environmental changes in Methylophilus methylotrophus and methods of producing amino acids in microorganisms having enhanced or attenuated expression of these polypeptides and/or polynucleotides.

1 . An isolated polynucleotide which encodes a protein comprising the amino acid sequence of SEQ ID NO:18.  
     
     
         2 . A vector comprising the isolated polynucleotide of  claim 1 .  
     
     
         3 . A host cell comprising the isolated polynucleotide of  claim 1 .  
     
     
         4 . The host cell of  claim 3 , which is a  Methylophilus  bacterium.  
     
     
         5 . The host cell of  claim 4 , which is a  Methylophilus methylotrophus  bacterium.  
     
     
         6 . A method of producing at least one amino acid comprising 
 culturing the host cell of  claim 3  for a time and under conditions suitable for producing the amino acid; and    collecting the amino acid produced.    
     
     
         7 . The method of  claim 6 , wherein said at least one amino acid is an L-amino acid.  
     
     
         8 . The method of  claim 6 , wherein said host cell is a  Methylophilus  bacterium.  
     
     
         9 . The method of  claim 6 , wherein said host cell is a  Methylophilus methylotrophus  bacterium.  
     
     
         10 . An isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO: 17.  
     
     
         11 . A vector comprising the isolated polynucleotide of  claim 10 .  
     
     
         12 . A host cell comprising the isolated polynucleotide of  claim 10 .  
     
     
         13 . The host cell of  claim 12 , which is a  Methylophilus  bacterium.  
     
     
         14 . The host cell of  claim 12 , which is a  Methylophilus methylotrophus  bacterium.  
     
     
         15 . A method of producing at least one amino acid comprising 
 culturing the host cell of  claim 12  for a time and under conditions suitable for producing the amino acid; and    collecting the amino acid produced.    
     
     
         16 . The method of  claim 15 , wherein said at least one amino acid is an L-amino acid.  
     
     
         17 . The method of  claim 15 , wherein said host cell is a  Methylophilus  bacterium.  
     
     
         18 . The method of  claim 15 , wherein said host cell is a  Methylophilus methylotrophus  bacterium.  
     
     
         19 . An isolated polynucleotide, which hybridizes under stringent conditions to the isolated polynucleotides of  claim 10 .  
     
     
         20 . A vector comprising the isolated polynucleotide of  claim 19 .  
     
     
         21 . A host cell comprising the isolated polynucleotide of  claim 19 .  
     
     
         22 . A method of producing at least one amino acid comprising 
 culturing the host cell of  claim 21  for a time and under conditions suitable for producing the amino acid; and    collecting the amino acid produced.    
     
     
         23 . The method of  claim 22 , wherein said at least one amino acid is an L-amino acid.  
     
     
         24 . The method of  claim 22 , wherein said host cell is a  Methylophilus  bacterium.  
     
     
         25 . The method of  claim 22 , wherein said host cell is a  Methylophilus methylotrophus  bacterium.  
     
     
         26 . An isolated polynucleotide, which is at least 95% identical to the polynucleotide of  claim 10 .  
     
     
         27 . A vector comprising the isolated polynucleotide of  claim 26 .  
     
     
         28 . A host cell comprising the isolated polynucleotide of  claim 26 .  
     
     
         29 . A method of producing at least one amino acid comprising 
 culturing the host cell of  claim 28  for a time and under conditions suitable for producing the amino acid; and    collecting the amino acid produced.    
     
     
         30 . The method of  claim 29 , wherein said at least one amino acid is an L-amino acid.  
     
     
         31 . The method of  claim 29 , wherein said host cell is a  Methylophilus  bacterium.  
     
     
         32 . The method of  claim 29 , wherein said host cell is a  Methylophilus methylotrophus  bacterium.