Embryonic stem cells capable of differentiating into desired cell lines

Abstract

An embryonic stem cell which may be induced to differentiate homogeneously into a desired primary cell line. The embryonic stem cell may be engineered with DNA, which encodes a protein or polypeptide which promotes differentiation of the stem cell into a specific cell line, such as, for example, a neuronal cell line, a muscle cell line, or a hematopoietic cell line. The DNA may encode a transcription factor found in the particular cell line. In another alternative, a desired cell line is produced from embryonic stem cells by culturing embryonic stem cells under conditions which provide for a three-dimensional network of embryonic stem cells, and then stimulating embryonic stem cells with an agent, such as retinoic acid, or dimethylsulfoxide, which promotes differentiation of the embryonic stem cells into the desired cell line, such as, for example, a neuronal cell line, or a muscle cell line.

1 - 15 . (canceled)  
     
     
         16 . An embyonic stem cell, said embryonic stem cell having been engineered with DNA which encodes a protein or polypeptide which promotes differentiation of said cell into a specific cell line wherein the cell is a neuronal cell line or a hematopoietic cell line.  
     
     
         17 . The cell of  claim 16  wherein said DNA which encodes a protein or polypeptide which promotes differentiation of said cell into a specific cell line is DNA encoding a transcription factor present in said specific cell line to promote differentiation of said cell into said specific cell line.  
     
     
         18 . The cell of  claim 17  wherein said DNA encoding a transcription factor is DNA encoding a transcription factor present in neuronal cells, and said specific cell line is a neuronal cell line.  
     
     
         19 - 29 . (canceled)