Nucleic acid fragments encoding proteins involved in stress response

Abstract

The invention provides isolated peptide-methionine sulfoxide reductase nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering peptide-methionine sulfoxide reductase levels in plants. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions.

1 . An isolated nucleic acid encoding a polypeptide selected from the group consisting of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, and 208.    
     
     
         2 - 25 . (canceled)  
     
     
         26 . An isolated polynucleotide comprising: 
 (a) a nucleotide sequence encoding a polypeptide having anthranilate N-hydroxycinnamoyl/benzoyltransferases activity, wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:52 have at least 80% sequence identity based on the Clustal alignment method with default pairwise alignment parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, or    (b) the complement of the nucleotide sequence of (a).    
     
     
         27 . The polynucleotide of  claim 26 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:52 have at least 85% sequence identity based on the Clustal alignment method with the default pairwise alignment parameters.  
     
     
         28 . The polynucleotide of  claim 26 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:52 have at least 90% sequence identity based on the Clustal alignment method with the default pairwise alignment parameters.  
     
     
         29 . The polynucleotide of  claim 26 , wherein the amino acid sequence of the polypeptide and the amino acid sequence of SEQ ID NO:52 have at least 95% sequence identity based on the Clustal alignment method with the default pairwise alignment parameters.  
     
     
         30 . The polynucleotide of  claim 26 , wherein the nucleotide sequence comprises the nucleotide sequence of SEQ ID NO:51.  
     
     
         31 . The polynucleotide of  claim 26 , wherein the amino acid sequence of the polypeptide comprises the amino acid sequence of SEQ ID NO:52.  
     
     
         32 . A vector comprising the polynucleotide of  claim 26 .  
     
     
         33 . A recombinant DNA construct comprising the polynucleotide of  claim 26  operably linked to at least one regulatory sequence.  
     
     
         34 . A method for transforming a cell comprising transforming a cell with the polynucleotide of  claim 26 .  
     
     
         35 . A cell comprising the recombinant DNA construct of  claim 33 .  
     
     
         36 . A method for producing a plant comprising transforming a plant cell with the polynucleotide of  claim 26  and regenerating a plant from the transformed plant cell.  
     
     
         37 . A plant comprising the recombinant DNA construct of  claim 33 .  
     
     
         38 . A seed comprising the recombinant DNA construct of  claim 33.