Proteolytic fermenter

Abstract

A process of controlling the production of enzymatic protein hydrolysate from cold water animals, containing up to 90% free amino acids, cold fish oil, hydroxilaptite (calcium orthophosphate), proteins, peptides, vitamin, minerals and calcium salts of phosphorus. The process provides a greater degree of refining than existing technology through the use of acid and alkaline denaturation steps, in combination with advanced separation and drying technology. The new drying technology results in a higher-quality, less expensive mixture than was previously possible.

1 . A method for producing a profound enzymatic protein hydrolysate, comprising: 
 preparing ferments and substrate made from cold water marine animals;    feeding said ferments and said substrate, water, and an alkaline solution to a piscean bioreactor;    heating and stirring the contents of said bioreactor to form a fish hydrolysate;    purifying said hydrolysate; and    concentrating said hyrolysate so as to produce an EPH comprising 70 to 90% free amino acids, 10 to 20% highly molecular peptides, and a remainder of nutrients.    
   
   
       2 . The method of  claim 1 , wherein said ferments are the viscera and internal organs of fish.  
   
   
       3 . The method of  claim 2 , wherein the mass content of said ferments are equivalent to the mass contents of said substrate.  
   
   
       4 . The method of  claim 3 , wherein said marine animals are farmed fish of one species.  
   
   
       5 . The method of  claim 3 , wherein said marine animals are selected from among the group consisting of cod, mackerel, and herring.  
   
   
       6 . The method of  claim 3 , wherein said marine animals are pelagic fish.  
   
   
       7 . The method of  claim 3 , wherein said marine animals are blue mussels.  
   
   
       8 . The method of  claim 4 , wherein said marine animals are salmon.  
   
   
       9 . The method of  claim 4 , wherein said marine animals are Atlantic krill.  
   
   
       10 . The method of  claim 4 , wherein said marine animals are tappai.  
   
   
       11 . The method of  claim 4 , wherein said marine animals are halibut.  
   
   
       12 . The method of  claim 4 , wherein said ferments are derived from a single organ.  
   
   
       13 . A method for producing an enzymatic protein hydrolysate, comprising: 
 feeding raw materials into a feed processor having a multi-ton capacity;    cutting up and mixing said raw materials in said feed processor to form a slurry;    feeding said slurry into an alkaline-based reactor to form a hydrolysate;    centrifuging said hydrolysate to produce an hydroxylapatite and a fish protein mix;    acid denaturating the fish protein mix to form an acid denaturated fish protein mix;    centrifuging the denaturated fish protein mix to produce cold fish oil and fish protein;    alkaline denaturating and centrifuging said fish protein to produce a peptide mix and phosphoric salts of calcium;    preserving the peptide mix with a mild acid; and    concentrating the final product to form an EPH that primarily includes free amino acids.    
   
   
       14 . The method of  claim 13 , wherein said EPH comprises the following amino acids: ______  
   
   
       15 . The method of  claim 13 , wherein concentrating the final product includes nutrient separation and drying.  
   
   
       16 . The method of  claim 15 , wherein nutrient separation is performed by a technique selected from among the group consisting of piezo-dialysis, ultrafiltration, and countercurrent extraction  
   
   
       17 . The method of  claim 16 , wherein nutrient separation results in a first product essentially consisting of free amino acids, a second product consisting of other nutrients.  
   
   
       18 . A computer method for establishing the quantity of water to be used in a piscean bioreactor, comprising: 
 inputing into a central control system raw material identifiers, comprising: 
 a substrate fish type and its mass;  
 a ferment source and its mass;  
   storing said raw material identifiers; and    outputing onto a display panel the mass of water required to operate the piscean bioreactor.    
   
   
       19 . A computer method for preparing raw materials to be used in a piscean bioreactor, comprising: 
 inputing into a central control system raw material identifiers, comprising: 
 a substrate fish type and its mass;  
 a ferment source and its mass;  
   storing said raw material identifiers; and    outputting the mass content of water required to operate the bioreactor.    
   
   
       20 . A computerized method for operation of a piscean bioreactor, comprising: 
 inputing raw material identifiers, comprising:    selecting the type of fish used;    inputing the total weight of raw materials to be used;    storing raw material identifiers;    receiving the appropriate quantity of water;    receiving pH for the bioreaction mixture;    receiving the temperature of the bioreaction mixture;    receiving the bioreaction time;    receiving the concentration of amino nitrogen in the bioreaction mixture;    receiving the pressure of the bioreaction vessel;    adjusting the pH of the bioreaction mixture;    adjusting the temperature of the bioreaction mixture;    adjusting pressure relief valves of the bioreactor; and    adjusting status indication for the bioreactor.