Bioarray chip reaction apparatus and its manufacture

Abstract

A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.

1 - 92 . (canceled)  
     
     
         93 . A method of aligning an array hybridization apparatus comprising: (a) providing a first substrate, second substrate and at least 2 standoffs; and (b) contacting the first substrate to the standoff to align the array hybridization apparatus.  
     
     
         94 . A method of making an array hybridization apparatus with a standoff for alignment, comprising: (a) providing a first substrate opposite a second substrate; (b) interposing a standoff between a first substrate and a second substrate; (c) contacting the first substrate and second substrate to the standoff.  
     
     
         95 . A method of making an array hybridization apparatus for aligning a gasket, comprising: (a) providing a first substrate; (b) attaching at least two standoffs to the first substrate; (c) using the standoff to align at least one gasket on the first substrate using the standoff and the gasket.