Method of producing a plurality of isolated antibodies to a plurality of cognate antigens

Abstract

The present invention relates to a method for producing high affinity antibodies that are antigen-specific. The method involves binding a plurality of antibody-producing B-cells from a mammal to a plurality of cognate antigens; sorting the bound antibody-producing B-cell and cognate antigen; amplifying nucleic acid sequences encoding each antibody, or fragment thereof, from the B-cells; and expressing the each antibody in a protein expression system. Antibodies produced in this manner are useful in diagnostic and therapeutic applications.

1 . A method for producing a plurality of isolated antibodies to a plurality of cognate antigens comprising 
 binding a plurality of antibody-producing B-cells from a mammal to a plurality of cognate antigens;    isolating each bound antibody-producing B-cell and cognate antigen;    amplifying nucleic acid sequences encoding each antibody, or fragment thereof, from the B-cells;    introducing each nucleic acid sequence encoding each antibody, or fragment thereof, into an expression system capable of expressing an antibody so that a plurality of isolated antibodies to a plurality of cognate antigens is produced.    
   
   
       2 . An antibody, or fragment thereof identified by the method of  claim 1.