US2006051829A1PendingUtilityA1

Regulating PAS domain function with foreign PAS ligands

Assignee: UNIV TEXASPriority: Jan 26, 2001Filed: Oct 11, 2005Published: Mar 9, 2006

Est. expiryJan 26, 2021(expired)· nominal 20-yr term from priority

Inventors:Kevin H. Gardner Carlos Amezcua Paulus Erbel Paul Card Shannon Harper Jared Rutter Richard Bruick Steven L. Mcknight

C12Q 1/485C12N 9/12G01N 33/573G01N 2500/20G01N 2500/00

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References

Claims

Abstract

A functional surface binding specificity of a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, is changed by (a) introducing into the hydrophobic core of the PAS domain a foreign ligand of the PAS domain; and (b) detecting a change in the functional surface binding specificity of the PAS domain.

Claims

exact text as granted — not AI-modified

1 . A method of changing a functional surface binding specificity of a PAS domain, wherein the PAS domain is predetermined, prefolded in its native state, and comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity, the method comprising the steps of: 
 introducing into the hydrophobic core of the PAS domain a foreign ligand of the PAS domain; and    detecting a resultant change in the functional surface binding specificity of the PAS domain,    wherein the PAS domain is PAS kinase PAS A, and the binding specificity is manifested as a change in kinase activity.    
   
   
       2 . The method of  claim 1 , wherein the PAS domain is part of PAS kinase.

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