US2006053505A1PendingUtilityA1

Bioluminescent novelty items

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Assignee: BRYAN BRUCEPriority: Feb 6, 1996Filed: Aug 24, 2005Published: Mar 9, 2006
Est. expiryFeb 6, 2016(expired)· nominal 20-yr term from priority
Inventors:Bruce Bryan
B05B 9/0833C12C 12/006C12N 15/8212A23L 17/30F21W 2131/401F41B 9/004Y10S493/955A23L 17/00C07K 14/43595A23P 10/30B05B 7/0408F21W 2121/02A23G 3/50F21V 33/0028C12C 12/004A23G 3/366F21K 2/06A61K 2800/434A61Q 19/00B05C 17/00553F21K 2/00A23K 20/00A24D 3/14F21S 8/00A23L 29/06A61Q 11/00A23G 9/363C12N 9/0071A23K 20/189B05B 7/2472A45D 34/00C12N 9/0069A23L 29/065A61K 8/64A23L 2/58Y02A40/818A23K 50/80A23V 2002/00A23G 9/44A23L 2/38C12G 1/06B05B 7/2416
50
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Claims

Abstract

Novelty items that are combinations of articles of manufacture with fluorescent proteins are provided. These novelty items, include combinations of transgenic plants that express a luciferase or a luciferin with plant food that contains a luciferase and a luciferin.

Claims

exact text as granted — not AI-modified
1 . A combination, comprising: 
 a transgenic fish that expresses one or more components of a bioluminescent system; and    a composition that includes one or more components of a bioluminescent system, wherein introducing the composition to the transgenic fish causes the transgenic fish to illuminate.    
     
     
         2 . The combination of  claim 1 , wherein the transgenic fish is an ornamental fish.  
     
     
         3 . The combination of  claim 1 , wherein the transgenic fish is a fishing lure or bait.  
     
     
         4 . The combination of  claim 1 , wherein the transgenic fish is substantially translucent.  
     
     
         5 . The combination of  claim 1 , wherein the transgenic fish is substantially transparent.  
     
     
         6 . The combination of  claim 1 , wherein the transgenic fish has cells and expresses one or more components of a bioluminescent system on the surfaces of the cells.  
     
     
         7 . The combination of  claim 1 , wherein the transgenic fish has mouth and a digestive system and expresses one or more components of a bioluminescent system on at least one of: the mouth or digestive system.  
     
     
         8 . The combination of  claim 1 , wherein the components of a bioluminescent system comprise at least one of the following: luciferase, luciferin, and fluorescent protein.  
     
     
         9 . The combination of  claim 8 , wherein the luciferase or luciferin is a  Renilla  luciferase or luciferin.  
     
     
         10 . The combination of  claim 8 , wherein the luciferase or luciferin is a Vargula luciferase or luciferin.  
     
     
         11 . The combination of  claim 8 , wherein the fluorescent protein is a green fluorescent protein.  
     
     
         12 . The combination of  claim 11 , wherein the green fluorescent protein is derived from the Aequorea fluorescent protein.  
     
     
         13 . The combination of  claim 11 , wherein the green fluorescent protein is derived from the  Renilla  fluorescent protein.  
     
     
         14 . The combination of  claim 8 , wherein the fluorescent protein is a blue fluorescent protein.  
     
     
         15 . The combination of  claim 14 , wherein the blue fluorescent protein is derived from  Vibrio  species fluorescent protein.  
     
     
         16 . The combination of  claim 1 , wherein the components of the bioluminescent system are derived from bacterial, fungal, insect, invertebrate or vertebrate bioluminescent systems.  
     
     
         17 . The combination of  claim 1 , wherein the transgenic fish emits sufficient illumination to be viewed by the unassisted eye.  
     
     
         18 . The combination of  claim 1 , wherein the transgenic fish emits illumination of a plurality of colors.  
     
     
         19 . The combination of  claim 1 , wherein the composition is a fish food.  
     
     
         20 . The combination of  claim 1 , wherein ingestion of the fish food by the fish causes the fish to illuminate.  
     
     
         21 . The combination of  claim 1 , wherein the transgenic fish is contained in water and the composition can be added directly to the water.  
     
     
         22 . A transgenic fish that expresses at least one component of a bioluminescent system.  
     
     
         23 . The transgenic fish of  claim 22 , wherein the transgenic fish is an ornamental fish.  
     
     
         24 . The transgenic fish of  claim 22 , wherein the transgenic fish is a fishing lure or bait.  
     
     
         25 . The transgenic fish of clam  22 , wherein the transgenic fish is substantially translucent.  
     
     
         26 . The transgenic fish of  claim 22 , wherein the transgenic fish is substantially transparent.  
     
     
         27 . The transgenic fish of  claim 22 , wherein the transgenic fish has cells and expresses one or more components of a bioluminescent system on the surfaces of the cells.  
     
     
         28 . The transgenic fish of  claim 22 , wherein the transgenic fish has a mouth and a digestive system and expresses one or more components of a bioluminescent system on at least one of the mouth or digestive system.  
     
     
         29 . The transgenic fish of  claim 22 , wherein the components of a bioluminescent system comprise at least one of the following: luciferase, luciferin, and fluorescent protein.  
     
     
         30 . The transgenic fish of  claim 22 , wherein the components of the bioluminescent system are derived from bacterial, fungal, insect, invertebrate of vertebrate bioluminescent systems.  
     
     
         31 . The transgenic fish of  claim 22 , wherein the transgenic fish emits sufficient illumination to be viewed by the unassisted eye.  
     
     
         32 . The transgenic fish of  claim 22 , wherein the transgenic fish emits illumination of a plurality of colors.  
     
     
         33 . A fish food that includes one or more components of a bioluminescent generating system.  
     
     
         34 . The fish food of  claim 33 , wherein the components of a bioluminescent generating system comprise at least one of: luciferase, luciferin, or fluorescent protein.  
     
     
         35 . The fish food of  claim 33 , wherein the luciferase or luciferin comprises from about 1 part per million to about 1 part per 10 weight/weight.  
     
     
         36 . The fish food of  claim 33 , wherein the luciferase or luciferin is in lyophilized form.  
     
     
         37 . A transgenic fish egg that expresses one or more components of a bioluminescent system.  
     
     
         38 . A method of making a transgenic fish emit illuminate, comprising: 
 providing a transgenic fish that expresses one or more components of a bioluminescent system; and    contacting the transgenic fish with a composition that comprises one or more components of a bioluminescent system, wherein contacting the transgenic fish with the composition causes the transgenic fish to illuminate.    
     
     
         39 . The method of  claim 38 , wherein the composition is a fish food.  
     
     
         40 . The method of  claim 38 , wherein the contacting the transgenic fish with a composition is consumption by the fish of the composition.  
     
     
         41 . A method of producing a transgenic fish, comprising: 
 providing fish eggs; and    transfecting the eggs with at least one luciferase or luciferin.    
     
     
         42 . The method of  claim 41 , wherein the eggs further comprise DNA endoding a membrane-spanning protein, and transfecting the eggs further includes incorporating one or more components of a bioluminescent system into the DNA encoding a membrane-spanning protein.  
     
     
         43 . A method of producing a transgenic fish food, comprising: 
 providing a fish food; and    including in the fish food one or more components of a bioluminescent system.

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