Transgenic plants incorporating traits of Zostera marina

Abstract

The invention provides methods and compositions related to transgenic plants which incorporate genetic traits of the marine eelgrass Zostera marina . These traits include pathogen resistance, which may be conferred by stimulating zosteric acid biosynthesis, and root anoxia resistance, which may be conferred by introducing one or more anoxia-induced or anoxia-resistance genes.

1 - 20 . (canceled)  
     
     
         21 . An isolated nucleic acid which hybridizes under stringent conditions including a wash step in 0.2×SSC at 65° C. to a nucleic acid consisting of the nucleotide sequence set forth in SEQ ID NO: 35, SEQ ID NO: 41, or SEQ ID NO: 46, wherein the isolated nucleic acid encodes a protein having alcohol dehydrogenase, cinnamate 4-hydroxylase, or phenylalanine ammonia lyase activity, respectively.  
     
     
         22 . An isolated nucleic acid encoding a polypeptide comprising an amino acid sequence having at least 95% identity with SEQ ID NO: 36, SEQ ID NO: 42, or SEQ ID NO: 47, wherein the polypeptide has alcohol dehydrogenase, cinnamate 4-hydroxylase, or phenylalanine ammonia lyase activity, respectively.  
     
     
         23 . The isolated nucleic acid of  claim 22 , encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 36, SEQ ID NO: 42, or SEQ ID NO: 47.  
     
     
         24 . The isolated nucleic acid of  claim 22 , encoding a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 36, SEQ ID NO: 42, or SEQ ID NO: 47.  
     
     
         25 . The isolated nucleic acid of  claim 21 , wherein the nucleic acid comprises the nucleotide sequence set forth in SEQ ID NO: 35, SEQ ID NO: 41, or SEQ ID NO: 46.  
     
     
         26 . The isolated nucleic acid of  claim 21  consisting of the nucleotide sequence set forth in SEQ ID NO: 35, SEQ ID NO: 41, or SEQ ID NO: 46.  
     
     
         27 . A vector comprising the nucleic acid of  claim 22 .  
     
     
         28 . A vector comprising the nucleic acid of  claim 21 .  
     
     
         29 . An expression vector comprising the nucleic acid of  claim 22 .  
     
     
         30 . An expression vector comprising the nucleic acid of  claim 21 .  
     
     
         31 . A cell comprising the vector of  claim 29 .  
     
     
         32 . The cell of  claim 31 , which is a prokaryotic cell.  
     
     
         33 . The cell of  claim 31 , which is an eukaryotic cell.  
     
     
         34 . A cell comprising the vector of  claim 30 .  
     
     
         35 . The cell of  claim 34 , which is a prokaryotic cell.  
     
     
         36 . The cell of  claim 34 , which is an eukaryotic cell.  
     
     
         37 . A plant comprising a heterologous nucleic acid of  claim 22 .  
     
     
         38 . The plant of  claim 37 , wherein the plant is a monocotyledon.  
     
     
         39 . The plant of  claim 37 , wherein the plant is a dicotyledon.  
     
     
         40 . A plant comprising a heterologous nucleic acid of  claim 21 .  
     
     
         41 . The plant of  claim 40 , wherein the plant is a monocotyledon.  
     
     
         42 . The plant of  claim 40 , wherein the plant is a dicotyledon.