Method of breeding glyphosate resistant plants

Abstract

Methods and compositions relating to glyphosate resistant maize plants, including the GA21, GG25, GJ11 and FI117 transformation events, are disclosed. Also disclosed are methods of using herbicide resistance transformation events in plant breeding procedures. The invention further includes methods of ensuring plant seed purity.

1 - 100 . (canceled)  
     
     
         101 . A method of plant breeding comprising the steps: 
 (i) planting a seed capable of growing into a first plant, said plant comprising a transformation event conferring herbicide resistance;    (ii) cultivating said seed to produce said first plant;    (iii) treating said first plant with a preselected herbicide to render pollen not having said transformation event inviable;    (iv) allowing pollen having said transformation event to pollinate said first plant or a second plant, wherein said pollen having said transformation event remains viable following said treating; and    (v) collecting seed from the first or the second plant.    
     
     
         102 . (canceled)  
     
     
         103 . (canceled)  
     
     
         104 . The method of  claim 101 , wherein said first plant is a maize plant.  
     
     
         105 . (canceled)  
     
     
         106 . (canceled)  
     
     
         107 . The method of  claim 101 , wherein said treating takes place between the V4 and VT stages of development.  
     
     
         108 - 118 . (canceled)  
     
     
         119 . A method of producing transgenic seed or transgenic progeny plants which have resistance to glyphosate, said method comprising: 
 (i) applying said glyphosate to a population of plants, at least some of said plants being heterozygous for a glyphosate resistance transgene, said applying being at an advanced vegetative state before flowering, wherein the glyphosate effectively eliminates the viability of the male gametes which do not carry the glyphosate resistance transgene, wherein the surviving male gametes which are capable of fertilizing female gametes carry the glyphosate resistance transgene; and    (ii) producing glyphosate resistant transgenic seed or transgenic plant progeny from said seed or plant population wherein the plant progeny are homozygous or heterozygous for the glyphosate resistance transgene.    
     
     
         120 . The method according to  claim 119  wherein the plants comprise crop plants.  
     
     
         121 . The method according to  claim 120  wherein the crop plants comprise corn.  
     
     
         122 . The method according to  claim 120  wherein in step (i), the herbicide is applied at the V5 stage of growth or later.  
     
     
         123 . The method according to  claim 122  wherein in step (i), the herbicide is applied between the V5 and V16 stages of growth.  
     
     
         124 . The method according to  claim 119  wherein the seed are hybrid seed.  
     
     
         125 . The method according  claim 119  wherein the plants contain a second transgene.  
     
     
         126 . The method according to  claim 125  wherein the second transgene controls fertility.  
     
     
         127 . The method according to  claim 125  wherein said second transgene controls male fertility.  
     
     
         128 . The method according to  claim 119  wherein said seed are inbred seed.  
     
     
         129 . A method of producing transgenic hybrid progeny plants which show resistance to glyphosate, said method comprising: 
 (i) applying said glyphosate to a population of progenitor plants, at least some of said progenitor plants being heterozygous for a glyphosate resistance transgene, such herbicide application being applied at an advanced vegetative state before flowering, wherein the glyphosate effectively eliminates the viability of the male gametes which do not carry the glyphosate resistance transgene, wherein the surviving male gametes which are capable of fertilizing female gametes carry the glyphosate resistance transgene, such that resultant male gametes from said plants fertilize inbred female plants which are homozygous or heterozygous for the glyphosate resistance transgene; and    (ii) producing glyphosate resistant transgenic hybrid seed or transgenic plant progeny from said inbred female plants wherein the seed or plant progeny are homozygous or heterozygous for the glyphosate resistance transgene.    
     
     
         130 . A method of producing transgenic seed or transgenic progeny plants which have resistance to glyphosate, said method comprising: 
 (i) applying said glyphosate to a population of plants, at least some of said plants being hemizygous for a glyphosate resistance transgene, said applying being before flowering, wherein the glyphosate effectively eliminates the viability of the male gametes which do not carry the glyphosate resistance transgene, wherein the surviving male gametes which are capable of fertilizing female gametes carry the glyphosate resistance transgene; and    (ii) producing glyphosate resistant transgenic seed or transgenic plant progeny from said seed or plant population wherein the plant progeny are homozygous or hemizygous for the glyphosate resistance transgene.    
     
     
         131 . The method according to  claim 130  wherein the plants comprise crop plants.  
     
     
         132 . The method according to  claim 131  wherein the crop plants comprise corn.  
     
     
         133 . The method according to  claim 130  wherein in step (i), the herbicide is applied at the V5 stage of growth or later.  
     
     
         134 . The method according to  claim 133  wherein in step (i), the herbicide is applied between the V5 and V16 stages of growth.  
     
     
         135 . The method according to  claim 130  wherein the seed are hybrid seed.  
     
     
         136 . The method according  claim 130  wherein the plants contain a second transgene.  
     
     
         137 . The method according to  claim 136  wherein the second transgene controls fertility.  
     
     
         138 . The method according to  claim 136  wherein said second transgene controls male fertility.  
     
     
         139 . The method according to  claim 130  wherein said seed are inbred seed.  
     
     
         140 . A method of producing transgenic hybrid progeny plants which show resistance to glyphosate, said method comprising: 
 (i) applying said glyphosate to a population of progenitor plants, at least some of said progenitor plants being hemizygous for a glyphosate resistance transgene, such herbicide application being applied before flowering, wherein the glyphosate effectively eliminates the viability of the male gametes which do not carry the glyphosate resistance transgene, wherein the surviving male gametes which are capable of fertilizing female gametes carry the glyphosate resistance transgene, such that resultant male gametes from said plants fertilize inbred female plants which are homozygous or hemizygous for the glyphosate resistance transgene; and    (ii) producing glyphosate resistant transgenic hybrid seed or transgenic plant progeny from said inbred female plants wherein the seed or plant progeny are homozygous or hemizygous for the glyphosate resistance transgene.