Modulating plant sugar levels

Abstract

This document provides methods and materials related to plants having modulated (e.g., increased) levels of sugars (e.g., glucose, fructose, and/or sucrose). For example, this document provides plants having increased sugar levels as well as methods and materials for making plants and plant products having increased sugar levels.

1 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, Ceres clone SEQ ID NO:9, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 , wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said isolated nucleic acid.  
     
     
         2 . The method of  claim 1 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 85 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         3 . The method of  claim 1 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 90 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         4 . The method of  claim 1 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         5 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, and the Consensus sequence set forth in  FIG. 6 , wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said isolated nucleic acid.  
     
     
         6 . The method of  claim 5 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 85 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         7 . The method of  claim 5 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 90 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         8 . The method of  claim 5 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         9 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:4, and the Consensus sequence set forth in  FIG. 6 , wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said isolated nucleic acid.  
     
     
         10 . The method of  claim 9 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 85 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:4, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         11 . The method of  claim 9 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 90 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:4, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         12 . The method of  claim 9 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:4, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         13 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:4, wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said isolated nucleic acid.  
     
     
         14 . The method of  claim 13 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 85 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:4.  
     
     
         15 . The method of  claim 13 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 90 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:4.  
     
     
         16 . The method of  claim 13 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:4.  
     
     
         17 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence corresponding to SEQ ID NO:2, wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said isolated nucleic acid.  
     
     
         18 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence corresponding to SEQ ID NO:14, wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said isolated nucleic acid.  
     
     
         19 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence corresponding to the Consensus sequence set forth in  FIG. 6 , wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said isolated nucleic acid.  
     
     
         20 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell (a) a first isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:2, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 ; and (b) a second isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence corresponding to SEQ ID NO:14; wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said first isolated nucleic acid or said second isolated nucleic acid.  
     
     
         21 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell (a) a first isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:2, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 ; and (b) a second isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence corresponding to SEQ ID NO:14; wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said first isolated nucleic acid or said second isolated nucleic acid.  
     
     
         22 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell (a) a first isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:2, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, and SEQ ID NO:8; and (b) a second isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence corresponding to SEQ ID NO:14; wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said first isolated nucleic acid or said second isolated nucleic acid.  
     
     
         23 . A method of modulating the level of a sugar in a plant, said method comprising introducing into a plant cell (a) a first isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:2, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, and SEQ ID NO:8; and (b) a second isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence corresponding to SEQ ID NO:14; wherein a plant produced from said plant cell has a different sugar level as compared to a sugar level in a corresponding control plant that does not comprise said first isolated nucleic acid or said second isolated nucleic acid.  
     
     
         24 . The method of  claim 1 ,  5 ,  9 ,  13 ,  17 ,  18 ,  19 ,  20 ,  21 ,  22 , or  23 , wherein said different sugar level is an increased level of one or more sugars.  
     
     
         25 . The method of  claim 24 , wherein said sugar is glucose.  
     
     
         26 . The method of  claim 24 , wherein said sugar is fructose.  
     
     
         27 . The method of  claim 24 , wherein said sugar is sucrose.  
     
     
         28 . The method of  claim 24 , wherein said different sugar level is an increased level of glucose and fructose.  
     
     
         29 . The method of  claim 24 , wherein said different sugar level is an increased level of glucose, fructose, and sucrose.  
     
     
         30 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 ; and (b) growing a plant from said plant cell.  
     
     
         31 . The method of  claim 30 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 85 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         32 . The method of  claim 30 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 90 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         33 . The method of  claim 30 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         34 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, and the Consensus sequence set forth in  FIG. 6 ; and (b) growing a plant from said plant cell.  
     
     
         35 . The method of  claim 34 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 85 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         36 . The method of  claim 34 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 90 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         37 . The method of  claim 34 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         38 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:4, and the Consensus sequence set forth in  FIG. 6 ; and (b) growing a plant from said plant cell.  
     
     
         39 . The method of  claim 38 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 85 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:4, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         40 . The method of  claim 38 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 90 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:4, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         41 . The method of  claim 38 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:4, and the Consensus sequence set forth in  FIG. 6 .  
     
     
         42 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:4; and (b) growing a plant from said plant cell.  
     
     
         43 . The method of  claim 42 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 85 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:4.  
     
     
         44 . The method of  claim 42 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 90 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:4.  
     
     
         45 . The method of  claim 42 , wherein said isolated nucleic acid comprises a nucleic acid sequence encoding a polypeptide having 95 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:14, SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:4.  
     
     
         46 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence corresponding to SEQ ID NO:14; and (b) growing a plant from said plant cell.  
     
     
         47 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence corresponding to SEQ ID NO:2; and (b) growing a plant from said plant cell.  
     
     
         48 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell an isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence corresponding to the Consensus sequence set forth in  FIG. 6 ; and (b) growing a plant from said plant cell.  
     
     
         49 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell a first isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:2, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 , and a second isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence corresponding to SEQ ID NO:14; and (b) growing a plant from said plant cell.  
     
     
         50 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell a first isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:2, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, SEQ ID NO:8, and the Consensus sequence set forth in  FIG. 6 , and a second isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence corresponding to SEQ ID NO:14; and (b) growing a plant from said plant cell.  
     
     
         51 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell a first isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:2, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, and SEQ ID NO:8, and a second isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence corresponding to SEQ ID NO:14; and (b) growing a plant from said plant cell.  
     
     
         52 . A method of producing a plant having a modulated level of a sugar, said method comprising (a) introducing into a plant cell a first isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:5, SEQ ID NO:10, SEQ ID NO:6, SEQ ID NO:11, SEQ ID NO:4, SEQ ID NO:9, SEQ ID NO:2, SEQ ID NO:7, SEQ ID NO:12, SEQ ID NO:3, and SEQ ID NO:8, and a second isolated nucleic acid comprising a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence corresponding to SEQ ID NO:14; and (b) growing a plant from said plant cell.  
     
     
         53 . The method of  claim 30 ,  34 ,  38 ,  42 ,  46 ,  47 ,  48 ,  49 ,  50 ,  51 , or  52 , wherein said modulated level is an increased level of one or more sugars.  
     
     
         54 . The method of  claim 53 , wherein said sugar is glucose.  
     
     
         55 . The method of  claim 53 , wherein said sugar is fructose.  
     
     
         56 . The method of  claim 53 , wherein said sugar is sucrose.  
     
     
         57 . The method of  claim 53 , wherein said modulated level is an increased level of glucose and fructose.  
     
     
         58 . The method of  claim 53 , wherein said modulated level is an increased level of glucose, fructose, and sucrose.  
     
     
         59 . The method of  claim 1 ,  5 ,  9 ,  13 ,  17 ,  18 ,  19 ,  30 ,  34 ,  38 ,  42 ,  46 ,  47 , or  48 , wherein said isolated nucleic acid is operably linked to a regulatory region.  
     
     
         60 . The method of  claim 20 ,  21 ,  22 ,  23 ,  49 ,  50 ,  51 , or  52 , wherein said first isolated nucleic acid and said second isolated nucleic acid is operably linked to a regulatory region.  
     
     
         61 . The method of  claim 59  or  60 , wherein said regulatory region is a promoter.  
     
     
         62 . The method of  claim 61 , wherein said promoter is a cell-specific or tissue-specific promoter.  
     
     
         63 . The method of  claim 61 , wherein said promoter is a broadly expressing promoter.  
     
     
         64 . The method of  claim 63 , wherein said broadly expressing promoter is selected from the group consisting of p326, YP0158, YP0214, YP0380, PT0848, PT0633, YP0050, YP0144, and YP0190.  
     
     
         65 . The method of  claim 62 , wherein said tissue-specific promoter is a seed-specific promoter.  
     
     
         66 . The method of  claim 65 , wherein said seed-specific promoter is selected from the group consisting of the napin promoter, the Arcelin-5 promoter, the phaseolin gene promoter, the soybean trypsin inhibitor promoter, the ACP promoter, the stearoyl-ACP desaturase gene, the soybean α′ subunit of β-conglycinin promoter, the oleosin promoter, the 15 kD zein promoter, the 16 kD zein promoter, the 19 kD zein promoter, the 22 kD zein promoter, the 27 kD zein promoter, the Osgt-1 promoter, the beta-amylase gene promoter, and the barley hordein gene promoter.  
     
     
         67 . The method of  claim 62 , wherein said tissue-specific promoter is a root-specific promoter.  
     
     
         68 . The method of  claim 67 , wherein said root-specific promoter is selected from the group consisting of the root specific subdomains of the CaMV 35S promoter and the tobacco RD2 gene promoter.  
     
     
         69 . The method of  claim 62 , wherein said tissue-specific promoter is a non-seed fruit tissue promoter.  
     
     
         70 . The method of  claim 69 , wherein said non-seed fruit tissue promoter is selected from the group consisting of a polygalacturonidase promoter, the banana TRX promoter, and the melon actin promoter.  
     
     
         71 . The method of  claim 61 , wherein said promoter is a constitutive promoter.  
     
     
         72 . The method of  claim 71 , wherein said promoter is selected from the group consisting of 35S, p32449, and p13879.  
     
     
         73 . The method of  claim 61 , wherein said promoter is an inducible promoter.  
     
     
         74 . The method of  claim 1 ,  5 ,  9 ,  13 ,  17 ,  18 ,  19 ,  20 ,  21 ,  22 ,  23 ,  30 ,  34 ,  38 ,  42 ,  46 ,  47 ,  48 ,  49 ,  50 ,  51 , or  52 , wherein said plant is from a genus selected from the group consisting of  Abies, Agrostis, Allium, Alseodaphne, Anacardium, Ananus, Andropogon, Arachis, Apium, Arabidopsis, Aragrostis, Ascophyllum, Asparagus, Atropa, Avena, Beilschmiedia, Bixa, Brassica, Calendula, Capsicum, Carthamus, Chondrus, Chicorium, Cinnamomum, Citrus, Citrullus, Cocculus, Cocos, Coffea, Corylus, Cracilaria, Croton, Crypthecodinium, Cucumis, Cucurbita, Cunninghamia, Cuphea, Cynodon, Daucus, Dianthus, Duguetia, Elaeis, Enteromorpha, Euphoria, Festuca, Festulolium, Ficus, Fragaria, Fucus, Glaucium, Glycine, Glycyrrhiza, Gossypium, Haematococcus, Helianthus, Heterocallis, Hevea, Himanthalia, Hordeum, Hyoscyamus, Lactuca, Landolphia, Lemna, Linum, Litsea, Lolium, Lycopersicon, Lupinus, Majorana, Malus, Manihot, Medicago, Mentha, Musa, Nicotiana, Odontella, Olea, Oryza, Palmaria, Panicum, Pannesetum, Parthenium, Persea, Petunia, Phaseolus, Phleum, Phoenix, Picea, Pinus, Pistacia, Pisum, Poa, Populus sect., Porphyra, Prunus, Pyrus, Raphanus, Ricinus, Rosa, Rosmarinus, Rubus, Saccharum, Salix, Schizochytrium, Secale, Senecio, Sinapis, Solanum, Sorghum, Spinacia, Spirulina, Stephania, Triticum, Tagetes, Theobroma, Trifolium, Trigonella, Ulva, Undaria, Vaccinium, Vicia, Vigna, Vitis,  and  Zea.    
     
     
         75 . The method of  claim 1 ,  5 ,  9 ,  13 ,  17 ,  18 ,  19 ,  20 ,  21 ,  22 ,  23 ,  30 ,  34 ,  38 ,  42 ,  46 ,  47 ,  48 ,  49 ,  50 ,  51 , or  52 , wherein said plant is a species selected from  Ananus comosus, Arabidopsis thaliana, Brassica rapa, Brassica napus, Brassica oleracea, Bixa orellana, Calendula officinalis, Cinnamomum camphora, Coffea arabica, Glycine max, Glycyrrhiza glabra, Gossypium hirsutum, Gossypium herbaceum, Lactuca sativa, Lycopersicon esculentum, Mentha piperita, Mentha spicata, Musa paradisiaca, Oryza sativa, Parthenium argentatum, Rosmarinus officinalis, Solanum tuberosum, Theobroma cacao, Triticum aestivum, Vitis vinifera,  and  Zea mays.    
     
     
         76 . The method of  claim 1 ,  5 ,  9 ,  13 ,  17 ,  18 ,  19 ,  20 ,  21 ,  22 ,  23 ,  30 ,  34 ,  38 ,  42 ,  46 ,  47 ,  48 ,  49 ,  50 ,  51 , or  52 , wherein said plant is selected from the group consisting of alfalfa, amaranth, apple, beans (including kidney beans, lima beans, dry beans, green beans), broccoli, cabbage, carrot, castor bean, chick peas, cherry, chicory, chocolate, clover, coffee, cotton, cottonseed, crambe, eucalyptus, flax, grape, grapefruit, lemon, lentils, lettuce, linseed, mango, melon (e.g., watermelon, cantaloupe), mustard, orange, peanut, peach, pear, peas, pepper, plum, poplar, potato, rapeseed (high erucic acid and canola), safflower, sesame, soybean, spinach, strawberry, sugarbeet, sunflower, tea, tomato, banana, barley, date palm, field corn, garlic, millet, oat, oil palm, onion, pineapple, popcorn, rice, rye, sorghum, sudangrass, sugarcane, sweet corn, switchgrass, turf grasses, wheat, fir, pine, spruce, brown seaweeds, green seaweeds, red seaweeds, and microalgae.