Proteins encoded by polynucleic acids of porcine reproductive and respiratory syndrome virus (PRRSV)

Abstract

The present invention provides an isolated DNA sequence encoding, for example, at least one polypeptide selected from the group consisting of proteins encoded by one or more open reading frames (ORF's) of an Iowa strain of porcine reproductive and respiratory syndrome virus (PRRSV), specifically ISU-12, and the polypeptides encoded by the isolated DNA sequences. The present invention also concerns a vaccine comprising an effective amount of such a protein; methods of producing antibodies which specifically bind to such a protein; and methods of protecting a pig against a PRRSV, and treating a pig infected by a PRRSV.

1 . A DNA sequence encoding a porcine reproductive and respiratory syndrome virus (PRRSV) consisting of SEQ ID NO:______ (ISU-12).  
     
     
         2 . A DNA sequence encoding an open reading frame of the PRRSV of  claim 1  selected from the group consisting of nucleotides 191-7387 of SEQ ID NO:______ (ORF1a), nucleotides 7375-11757 of SEQ ID NO:______ (ORF 1b), nucleotides 11762-12529 of SEQ ID NO:______ (ORF 2), nucleotides 12385-13116 of SEQ ID NO:______ (ORF 3), nucleotides 12930-13463 of SEQ ID NO:______ (ORF 4), nucleotides 13477-14076 of SEQ ID NO:______ (ORF 5), nucleotides 14064-14585 of SEQ ID NO:______ (ORF 6) and nucleotides 14578-14946 of SEQ ID NO:______ (ORF 7).  
     
     
         3 . A polypeptide encoded by the DNA sequence of  claim 2 .  
     
     
         4 . A composition for inducing antibodies against PRRSV comprising one or more polypeptides encoded by the DNA sequences of  claim 2 .  
     
     
         5 . A DNA sequence encoding a PRRSV consisting of SEQ ID NO:______ (ISU-55).  
     
     
         6 . A DNA sequence encoding an open reading frame of the PRRSV of  claim 5  selected from the group consisting of nucleotides 191-7699 of SEQ ID NO:______ (ORF1a), nucleotides 7687-12069 of SEQ ID NO:______ (ORF 1b), nucleotides 12074-12841 of SEQ ID NO:______ (ORF 2), nucleotides 12692-13458 of SEQ ID NO:______ (ORF 3), nucleotides 13212-13775 of SEQ ID NO:______ (ORF 4), nucleotides 13789-14388 of SEQ ID NO:______ (ORF 5), nucleotides 14376-14592 of SEQ ID NO:______ (ORF 6) and nucleotides 14890-15258 of SEQ ID NO:______ (ORF 7).  
     
     
         7 . A polypeptide encoded by the DNA sequence of  claim 6 .  
     
     
         8 . A composition for inducing antibodies against PRRSV comprising an amount of a PRRSV encoded by SEQ ID NO:______ (ISU-55) effective to induce said antibodies in a pig, and a physiologically acceptable carrier.  
     
     
         9 . A composition for inducing antibodies against PRRSV comprising one or more polypeptides encoded by the DNA sequences of  claim 6 .  
     
     
         10 . The composition of  claim 8 , wherein lung lesions in said five-week-old colostrum-deprived, caesarean-derived pigs are reduced by a statistically significant amount wherein said amount is significant a p value less than 0.01, relative to lung lesions in uninoculated five-week-old colostrum-deprived, caesarean-derived pigs.  
     
     
         11 . The composition of  claim 9 , wherein lung lesions in said five-week-old colostrum-deprived, caesarean-derived pigs are reduced by a statistically significant amount wherein said amount is significant a p value less than 0.101, relative to lung lesions in uninoculated five-week-old colostrum-deprived, caesarean-derived pigs.  
     
     
         12 . The composition of  claim 8  further comprising an adjuvant.  
     
     
         13 . The composition of  claim 9  further comprising an adjuvant.  
     
     
         14 . A method of protecting a pig from a porcine reproductive and respiratory disease, comprising administering an effective amount of the composition of  claim 8  to a pig in need of protection against said disease.  
     
     
         15 . The method of  claim 14 , wherein said vaccine is administered orally or parenterally.  
     
     
         16 . The method of  claim 14 , wherein said vaccine is administered intramuscularly, intradermally, intravenously, intraperioneally, subcutaneously or intranasally.  
     
     
         17 . A method of protecting a pig from a porcine reproductive and respiratory disease, comprising administering an effective amount of the composition of  claim 9  to a pig in need of protection against said disease.  
     
     
         18 . The method of  claim 17 , wherein said vaccine is administered orally or parenterally.  
     
     
         19 . The method of  claim 17 , wherein said vaccine is administered intramuscularly, intradermally, intravenously, intraperitoneally, subcutaneously or intranasally.  
     
     
         20 . A method of distinguishing PRRSV strain ISU-55 from other strains of PRRSV comprising: 
 (a) amplifying a DNA sequence of the PRRSV using the following two primers:                                          55F   5′-CGTACGGCGATAGGGACACC-3′             and                   3RFLP   5′-GGCATATATCATCACTGGCG-3′;                                (b) digesting the amplified sequence of step (a) with DraI; and    (c) correlating the presence of three restriction fragments of 626 bp, 187 bp and 135 bp with a PRRSV ISU-55 strain.