Novel gene and protein encoded by the gene

Abstract

Novel DNAs containing the regions which encode proteins have been directly cloned from cDNA libraries derived from the human adult whole brain, the human adult hippocampus and the human embryonic whole brain, the nucleotide sequences thereof have been determined, and their functions have been identified. The present invention provides DNA which comprises the nucleotide sequence encoding the following polypeptide (a) or (b): (a) a polypeptide comprising an amino acid sequence which is identical or substantially identical to an amino acid sequence represented by any one of SEQ ID NOS: 1 to 70; (b) a polypeptide comprising an amino acid sequence derived from the amino acid sequence represented by any one of SEQ ID NOS: 1 to 70 by deletion, substitution or addition of a section of amino acids, and having biological activity which is substantially the same characteristic with the function of the polypeptide of (a); a recombinant polypeptide, which is encoded by the above DNA; and a protein containing the polypeptide.

1 . DNA comprising a nucleotide sequence encoding a polypeptide (a) or (b) as follows: 
 (a) a polypeptide comprising an amino acid sequence which is identical or substantially identical to an amino acid sequence represented by any one of SEQ ID NOS: 1 to 70;    (b) a polypeptide which comprises an amino acid sequence derived from an amino acid sequence represented by any one of SEQ ID NOS: 1 to 70 by deletion, substitution or addition of a section of amino acid(s), and has biological activity which is substantially the same characteristic with the function of the polypeptide of (a).    
     
     
         2 . DNA hybridizing to the DNA of  claim 1  under stringent conditions, and encoding a polypeptide having biological activity which is substantially the same characteristic with the function of the polypeptide of (a) of  claim 1 .  
     
     
         3 . A gene construct containing the DNA of  claim 1  or  2 .  
     
     
         4 . A polypeptide (a) or (b) as follows: 
 (a) a polypeptide comprising an amino acid sequence which is identical or substantially identical to an amino acid sequence represented by any one of SEQ ID NOS: 1 to 70;    (b) a polypeptide comprising an amino acid sequence derived from an amino acid sequence represented by any one of SEQ ID NOS: 1 to 70 by deletion, substitution or addition of a section of amino acids, and having biological activity which is substantially the same characteristic with the function of the polypeptide of (a).    
     
     
         5 . A recombinant polypeptide, which is encoded by the gene construct of  claim 3 .  
     
     
         6 . An antibody against the polypeptide of  claim 4  or  5 .  
     
     
         7 . A DNA chip, on which the DNAs of  claim 1  or  2  are arrayed.  
     
     
         8 . A polypeptide chip, on which the polypeptides of  claim 4  or  5  are arrayed.  
     
     
         9 . An antibody chip, on which the antibodies of  claim 6  are arrayed.