Therapeutic polypeptides, nucleic acids encoding same, and methods of use

Abstract

Disclosed herein are nucleic acid sequences that encode G-coupled protein-receptor related polypeptides. Also disclosed are polypeptides encoded by these nucleic acid sequences, and antibodies, which immunospecifically-bind to the polypeptide, as well as derivatives, variants, mutants, or fragments of the aforementioned polypeptide, polynucleotide, or antibody. The invention further discloses therapeutic, diagnostic and research methods for diagnosis, treatment, and prevention of disorders involving any one of these novel human nucleic acids and proteins.

1 . An isolated polypeptide comprising an amino acid sequenced selected from the group consisting of SEQ ID NO:2n, wherein n is an integer between 1 and 54  
     
     
         2 . An isolated polypeptide comprising a mature form of the amino acid sequence of  claim 1 .  
     
     
         3 . (canceled)  
     
     
         4 . An isolated polypeptide comprising an amino acid sequence having one or more conservative substitutions in the amino acid sequence of  claim 1 .  
     
     
         5 . (canceled)  
     
     
         6 . A composition comprising the polypeptide of  claim 1  and a carrier.  
     
     
         7 .- 19 . (canceled)  
     
     
         20 . An isolated nucleic acid molecule comprising a nucleic acid sequence encoding the polypeptide of  claim 1 .  
     
     
         21 . (canceled)  
     
     
         22 . A nucleic acid molecule, wherein the nucleic acid molecule differs by a single nucleotide from a nucleic acid sequence of  claim 20 .  
     
     
         23 . An isolated nucleic acid molecule encoding the polypeptide of  claim 2 .  
     
     
         24 . (canceled)  
     
     
         25 . (canceled)  
     
     
         26 . A vector comprising the nucleic acid molecule of  claim 20 .  
     
     
         27 . (canceled)  
     
     
         28 . A cell comprising the vector of  claim 26 .  
     
     
         29 . An antibody that immunospecifically binds to the polypeptide of  claim 1 .  
     
     
         30 . The antibody of  claim 29 , wherein the antibody is a monoclonal antibody.  
     
     
         31 . The antibody of  claim 29 , wherein the antibody is a humanized antibody.  
     
     
         32 .- 40 . (canceled)  
     
     
         41 . A method of producing the polypeptide of  claim 1 , the method comprising culturing a cell under conditions that lead to expression of the polypeptide, wherein said cell comprises a vector comprising an isolated nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO:2n-1, wherein n is an integer between 1 and 54.  
     
     
         42 .- 45 . (canceled)  
     
     
         46 . An isolated polypeptide comprising an amino acid sequence of SEQ ID NO: 48.  
     
     
         47 . The isolated polypeptide of  claim 46  consisting of amino acid sequence of SEQ ID NO: 48.  
     
     
         48 . An isolated polypeptide comprising an amino acid sequence having one or more conservative substitutions to SEQ ID NO: 48.  
     
     
         49 . An isolated polypeptide comprising an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 48.  
     
     
         50 . A pharmaceutical composition comprising the polypeptide of  claim 46 , and a pharmaceutically acceptable carrier.  
     
     
         51 . A pharmaceutical composition comprising the polypeptide of  claim 48 , and a pharmaceutically acceptable carrier.  
     
     
         52 . A pharmaceutical composition comprising the polypeptide of  claim 49 , and a pharmaceutically acceptable carrier.