Compositions and methods for treating angiogenesis-related diseases, wounds and cosmetic use of components of Angelica sinensis, and methods of preparation thereof

Abstract

Compositions and methods for treating angiogenesis-related diseases and for skin care in mammals is disclosed that includes, as an active pharmaceutical agent, an effective amount of purified extract from Angelica sinensis , or a fraction or a lyophilizate thereof, or one or more active component contained in said extract.

1 . A method of promoting angiogenesis in a mammalian tissue comprising directly contacting said tissue with an effective amount of  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract to promote angiogenesis in said tissue.  
   
   
       2 . The method of  claim 1 , wherein said tissue is selected from the group consisting of endothelial, cardiac, cerebral, muscular, vascular, bone, fat, transplanted, dermal, epidermal and wounded.  
   
   
       3 . The method of  claim 1 , wherein said tissue is an ischemic tissue selected from the group consisting of myocardial ischemic tissue, cerebral ischemic tissue, veno-occlusive diseased tissue, wounded tissue, damaged dermal tissue, damaged epidermal tissue, diabetic tissue and myocardial ischemic tissue wherein said myocardial ischemic tissue is implicated in coronary artery diseases.  
   
   
       4 . The method of  claim 1 , wherein said tissue to be promoted for angiogenesis is implicated in an ischemic disease.  
   
   
       5 . The method of  claim 1 , wherein said angiogenesis promotion is implicated in wound healing.  
   
   
       6 . The method of  claim 1 , wherein said angiogenesis promotion is implicated in prevention of scar formation.  
   
   
       7 . The method of  claim 3 , further comprises directly contacting said tissue with said effective amount of said  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract, in combination with a growth factor selected from the group consisting of fibroblast growth factors (FGF), FGF-1, FGF-2, FGF-4, thymosins, platelet-derived growth factors (PDGF), insulin-binding growth factors (IGF), IGF-1, IGF-2, epidermal growth factor (EGF), transforming growth factor (TGF), TGF-.alpha., TGF-.beta., cartilage inducing factors-A and -B, osteoid-inducing factors, osteogenin, bone morphogenic proteins, and other bone growth factors, collagen growth factors, heparin-binding growth factor-1 or -2, and their biologically active derivatives.  
   
   
       8 . The method of  claim 5 , further comprises directly contacting said tissue with said effective amount of said  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract, in combination with collagen.  
   
   
       9 . The method of  claim 5 , further comprises directly contacting said tissue with said effective amount of said  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract, in combination with polysaccharides.  
   
   
       10 . The method of  claim 5 , further comprises directly contacting said tissue with said effective amount of said  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract, in combination with skin substitutes.  
   
   
       11 . The method of  claim 5 , further comprises directly contacting said tissue with said effective amount of said  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract, in combination with antibiotics or other antimicrobial agents.  
   
   
       12 . The method in  claim 3 , wherein effective amount of said  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract is formulated in an edible power bar.  
   
   
       13 . The method of  claim 3 , further comprises directly contacting said tissue with a pharmaceutically acceptable formulation of  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract.  
   
   
       14 . The formulation of  claim 9 , wherein said formulation is packaged in a sachet.  
   
   
       15 . The formulation of  claim 9 , wherein said formulation is a potable liquid.  
   
   
       16 . The formulation of  claim 9 , wherein said formulation is a tablet or pill.  
   
   
       17 . The formulation of  claim 9 , wherein said formulation is injectable.  
   
   
       18 . The method of  claim 5 , further comprising a method of treating a wound with a dressing material, comprising: forming a gel with a gel forming amount of a water soluble or water-swellable pharmaceutically acceptable polymer, excipients and effective amount of  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract; dehydrating the gel to a preparation in the form of a foam or powder form of the gel material; attaching said preparation to a backing; said backing containing said preparation being applied to the wound.  
   
   
       19 . The method of  claim 18 , wherein said foam or powder is in a suitable form so that it will adhere to a wound for at least 24 hours and during that time, enhance healing of the wound and help prevent infection of the wound.  
   
   
       20 . The method of  claim 18  wherein the backing is an adhesive medical tape.  
   
   
       21 . The method of  claim 18  wherein the backing comprises a porous material.  
   
   
       22 . The method of  claim 18  wherein the backing comprises a non-porous material.  
   
   
       23 . The method of  claim 18  wherein the backing comprises a micro-porous material.  
   
   
       24 . The method of  claim 18  wherein the preparation is partially rehydrated to form a hydro-gel prior to application to the wound.  
   
   
       25 . The method of  claim 5 , further comprising a method of treating a wounded and otherwise damaged skin with an aqueous soaking solution containing SBD.4  
   
   
       26 . An acceptable formulation for cosmetic use as lotion, cream, cleanser and shampoo comprising effective amount of  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof.  
   
   
       27 . The formulation of  claim 26 , wherein said formulation contains a skin-soothing agent selected from the group consisting of dimethicone and allantoin.  
   
   
       28 . The formulation of  claim 26 , wherein said formulation contains a UV screening agent and a whitening agent.  
   
   
       29 . A method of promoting fibroblast growth in a mammalian tissue comprising contacting said tissue with an effective amount of  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract, to promote wound-healing in said tissue.  
   
   
       30 . The method of  claim 29 , wherein said fibroblast growth promotion is associated with increased collagen output.  
   
   
       31 . The method of  claim 29 , wherein said fibroblast growth promotion is implicated in wound healing.  
   
   
       32 . The method of  claim 29 , wherein said increased collagen output is implicated in wound healing.  
   
   
       33 . The method of  claim 29 , wherein said  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract, contains components selected from the group consisting of molecules with mass-to-charge (M/Z) ratio value of 147, 174, 218,216, 214, 304, 193, 250,307 and 406.  
   
   
       34 . The method of  claim 33 , wherein said active component is selected from the group consisting of molecules with mass-to-charge (M/Z) ratio value of 174.  
   
   
       35 . The method of  claim 33 , wherein said active component is a molecule with mass-to-charge (M/Z) ratio value of 218.  
   
   
       36 . The method of  claim 26 , wherein said  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract, contains components selected from the group consisting of molecules with mass-to-charge (M/Z) ratio value of 147, 174, 218, 216, 214,304, 193, 250,307 and 406.  
   
   
       37 . The method of  claim 36 , wherein said active component is a molecule with mass-to-charge (M/Z) ratio value of 174.  
   
   
       38 . The method of  claim 36 , wherein said active component is a molecule with mass-to-charge (M/Z) ration value of  218 .  
   
   
       39 . The method of  claim 1 , wherein said  Angelica sinensis  extract SBD.4 or a fraction or a lyophilizate thereof, or one or more active components contained in said extract, contains components selected from the group consisting of molecules with mass-to-charge (M/Z) ratio value of 147, 174, 218, 216, 214, 304, 193, 250, 307 and 406.  
   
   
       40 . The method of  claim 39 , wherein said active component is a molecule with mass-to-charge (M/Z) ratio value of 1 74.  
   
   
       41 . The method of  claim 39 , wherein said active component is a molecule with mass-to-charge (M/Z) ratio value of 218.