Extract of cercis chinensis having anti-oxidant activity and anti-aging activity, and cosmetical composition containing the extract for anti-oxidation, skin-aging protection and wrinkle improvement

Abstract

The present invention relates to extract of Cercis chinensis having anti-oxidant activity and antiaging activity containing compound of chemical formula 1 to chemical formula 20, and cosmetical composition for anti-oxidation, skin-aging protection and wrinkle improvement containing the extract as effective ingredient. The extract of present invention having protective effect on oxidative damage and skin damage, and inhibitory effect on age-dependent telomere shortening, so it can effectively used as skin-aging protection comestic.

1 . An extract of  Cercis chinensis  having activities of anti-oxidation, anti-skin aging, protecting skin elasticity and preventing wrinkles, extracted by using water or alcohol aqueous solution as an extractant.  
     
     
         2 . The extract as set forth in  claim 1 , wherein the alcohol aqueous solution is selected from a group consisting of methanol aqueous solution, ethanol aqueous solution, propanol aqueous solution and butanol aqueous solution.  
     
     
         3 . The extract as set forth in  claim 2 , wherein the alcohol aqueous solution is 50-80% ethanol aqueous solution.  
     
     
         4 . The extract as set forth in  claim 3 , wherein the alcohol aqueous solution is 60% ethanol aqueous solution.  
     
     
         5 . The extract as set forth in  claim 1 , wherein the extract contains one or more compounds selected from a group consisting of compounds represented by <Chemical Formula 1> (isoliquiritigenin), <Chemical Formula 2> (2′,4′-dihydroxy-4-methoxychalcone), <Chemical Formula 3> (liquiritigenin), <Chemical Formula 4> (resveratrol), <Chemical Formula 5> (piceatannol), <Chemical Formula 6> (gallic acid), <Chemical Formula 7> (methyl gallate), <Chemical Formula 8> (ethyl gallate), <Chemical Formula 9> (myricetin), <Chemical Formula 10> (afzelin), <Chemical Formula 11> (quercitrin), <Chemical Formula 12> (myricitrin), <Chemical Formula 13> (myricetin-3-O-(2″-O-galloyl)-α-L-rhamnopyranoside), <Chemical Formula 14> (syringetin-3-O-rutinoside), <Chemical Formula 15> (syringetin-3-O-2″-O-galloyl)-rutinoside), <Chemical Formula 16> ((+)-catechin), <Chemical Formula 17> ((−)-epicatechin-3-O-gallate), <Chemical Formula 18> ((−)-epigallocatechin-3-O-gallate), <Chemical Formula 19> ((−)-lyoniresinol 3a-O-β-D-xylopyranoside) and <Chemical Formula 20> ((+)-lyoniresiol 3a-O-β-D-glucopyranoside).  
     
     
         6 . The extract as set forth in  claim 5 , wherein the extract contains a compound represented by <Chemical Formula 6> by 0.01˜1.00 weight %, a compound represented by <Chemical Formula 12> 0.01˜1.00 weight % and a compound represented by <Chemical Formula 5> by 0.01˜0.5 weight %.  
     
     
         7 . A cosmetic composition for anti-oxidation, anti-skin aging, protecting skin elasticity or preventing wrinkles, which contains the extract of  claim 1  as an effective ingredient.  
     
     
         8 . A cosmetic composition for anti-oxidation, anti-skin aging, protecting skin elasticity or preventing wrinkles, which contains one or more compounds selected from a group consisting of compounds represented by <Chemical Formula 1> to <Chemical Formula 20> separated from the extract of  claim 1  as effective ingredients.  
     
     
         9 . The cosmetic composition as set forth in  claim 7 , wherein the cosmetic composition is selected from a group consisting of basic skin care cosmetics such as soft lotion, nutritive lotion, nutritive cream, essence, pack and bath powder.  
     
     
         10 . A pharmaceutical composition for anti-oxidation and anti-aging containing the extract of  Cercis chinensis  as an effective ingredient.  
     
     
         11 . The pharmaceutical composition as set forth in  claim 10 , wherein the pharmaceutical composition is prepared in the forms of injections, tablets or syrups.  
     
     
         12 . A preparation method of the extract of  Cercis chinensis  of  claim 1 , which is comprised of the following steps: 
 1) Crude-extracting of pulverized powder of  Cercis chinensis  using alcohol;    2) Extracting the alcohol crude extract of the above step 1) with hexane, ethylacetate, and butanol in that order;    3) Performing ethanol:water density gradient column chromatography with the ethylacetate fraction or butanol fraction obtained in the above step 2); and    4) Obtaining a final anti-oxidant extract by performing column chromatography, TLC or HPLC with the fraction having an anti-oxidant activity obtained in the above step 3).    
     
     
         13 . The preparation method as set forth in  claim 12 , wherein the alcohol is 60% ethanol.