Method for treatment of osteochondral and bone defects using an acellular matrix implant

Abstract

An acellular matrix implant for treatment of defects and injuries of articular cartilage, bone or osteochondral bone and a method for treatment of injured, damaged, diseased or aged articular cartilage or bone, using the acellular matrix implant implanted into a joint cartilage lesion in situ and a bone-inducing composition implanted into an osteochondral or bone defect. A method for repair and restoration of the injured, damaged, diseased or aged cartilage or bone into its full functionality by implanting the acellular matrix implant between two layers of biologically acceptable sealants and/or the bone-inducing composition into the osteochondral bone or skeletal bone defect. A method for fabrication of the acellular matrix implant of the invention. A method for preparation of bone-inducing composition.

1 . (canceled)  
   
   
       2 . (canceled)  
   
   
       3 . (canceled)  
   
   
       4 . (canceled)  
   
   
       5 . (canceled)  
   
   
       6 . (canceled)  
   
   
       7 . (canceled)  
   
   
       8 . (canceled)  
   
   
       9 . (canceled)  
   
   
       10 . (canceled)  
   
   
       11 . (canceled)  
   
   
       12 . (canceled)  
   
   
       13 . (canceled)  
   
   
       14 . (canceled)  
   
   
       15 . (canceled)  
   
   
       16 . A method for treatment of osteochondral defects, said method comprising steps: 
 a) preparing a bone-inducing composition or a carrier comprising said composition, said composition comprising one or several bone-inducing agents, for implantation into a bone lesion;    b) preparing an acellular matrix implant for implantation into a cartilage lesion as a collagenous sponge, collagenous porous scaffold, a polymer of an aromatic organic acid or thermo-reversible gelation hydrogel (TRGH) matrix support wherein said sponge, scaffold, polymer or TRGH are biodegradable, will disintegrate with time, be removed and replaced with a hyaline cartilage;    c) introducing said bone-inducing composition or a carrier comprising said composition into a bone lesion;    d) covering said bone-inducing composition or the carrier comprising said composition with a bottom sealant;    e) implanting said acellular matrix implant into said cartilage lesion over the bottom sealant; and    f) introducing a layer of a top sealant over said implant wherein said top and bottom sealants may or may not be the same.    
   
   
       17 . The method of  claim 16  wherein said inducing agent is selected from the group consisting of a demineralized bone powder, hydroxyapatite, organoapatite, calcium phosphate, titanium oxide, poly-L-lactic acid, polyglycolic acid, a copolymer thereof and a bone morphogenic protein.  
   
   
       18 . The method of  claim 17  wherein said bone-inducing agent is hydroxyapatite.  
   
   
       19 . The method of  claim 17  wherein said bone-inducing agent is the demineralized bone powder.  
   
   
       20 . The method of  claim 19  wherein said demineralized bone powder is dissolved in collagen.  
   
   
       21 . The method of claims  16  wherein said carrier comprising the bone-inducing agent is a polymer of an aromatic organic acid.  
   
   
       22 . A method for treatment of bone defects and fractures, said method comprising steps: 
 a) preparing a bone-inducing composition or an implant carrier comprising said composition, said composition comprising one or several bone-inducing agents for implantation into a bone lesion;    b) introducing said bone-inducing composition or said carrier comprising said composition into the bone lesion;    c) covering said bone-inducing composition or said carrier comprising said composition with a layer of a sealant.    
   
   
       23 . The method of  claim 22  wherein said bone-inducing agent is selected from the group consisting of a demineralized bone powder, hydroxyapatite, organoapatite, calcium phosphate, titanium oxide, poly-L-lactic acid, polyglycolic acid, a copolymer thereof and a bone morphogenic protein.  
   
   
       24 . The method of  claim 23  wherein said bone-inducing agent is the demineralized bone powder.  
   
   
       25 . The method of  claim 24  wherein said demineralized bone powder is mixed with collagen into a stable paste.  
   
   
       26 . The method of claims  23  wherein said carrier comprising said bone-inducing agent is a polymer of an organic aromatic acid.  
   
   
       27 . The method of  claim 23  wherein said bone-inducing agent is the hydroxyapatite.  
   
   
       28 . The method of  claim 23  wherein said bone-inducing composition is the bone morphogenic protein.  
   
   
       29 . The method of claims  23  wherein said bone-inducing agent is titanium oxide.  
   
   
       30 . (canceled)  
   
   
       31 . (canceled)  
   
   
       32 . (canceled)  
   
   
       33 . (canceled)  
   
   
       34 . (canceled)  
   
   
       35 . (canceled)  
   
   
       36 . (canceled)  
   
   
       37 . (canceled)