Gel having biosubstance fixed thereto and microarray utilizing the gel

Abstract

The present invention provides a biological substance-immobilized gel which comprises a gel containing 2%-7% by mass of N,N-dimethylacrylamide and a biological substance immobilized on and/or in the gel.

1 . A biological substance-immobilized gel which comprises a gel containing 2%-7% by mass of N,N-dimethylacrylamide and a biological substance immobilized on and/or in the gel.  
     
     
         2 . A biological substance-immobilized gel which comprises a gel having the following composition and a biological substance immobilized on and/or in the gel:  
       
         
           
                 
                 
                 
               
                     
                     
                 
                     
                     
                 
                     
                   (a) N,N-dimethylacrylamide 
                     2% to 7% by mass 
                 
                     
                   (b) cross-linking agent 
                   0.1% to 1.5% by mass. 
                 
                     
                     
                 
                     
                     
                 
             
                
                
               
               
                
                
                
                
               
            
           
         
       
     
     
         3 . The biological substance-immobilized gel according to  claim 1  or  2 , wherein the biological substance is a nucleic acid.  
     
     
         4 . The biological substance-immobilized gel according to  claim 2  or  3 , wherein the cross-linking agent is a multifunctional monomer having at least two ethylenically unsaturated bonds.  
     
     
         5 . The biological substance-immobilized gel according to  claim 4 , wherein the cross-linking agent is methylenebisacrylamide.  
     
     
         6 . A method for preparing a biological substance-immobilized gel, which comprises immobilizing a biological substance on and/or in a gel containing 2%-7% by mass of N,N-dimethylacrylamide.  
     
     
         7 . The method according to  claim 6 , wherein the gel is obtained by reacting 2%-7% by mass of N,N-dimethylacrylamide in the presence of 0.1%-1.5% by mass of a cross-linking agent.  
     
     
         8 . A gel-filled hollow tube which comprises a hollow tube whose hollow space is filled with the biological substance-immobilized gel according to any one of  claims 1  to  5 .  
     
     
         9 . The gel-filled hollow tube according to  claim 8 , wherein the hollow tube is a hollow fiber.  
     
     
         10 . A method for manufacturing a biological substance-immobilized gel microarray, which comprises allowing a plurality of gel-filled hollow tubes according to  claim 8  or  9  to be tied in a bundle and cutting the resulting tube bundle in a direction intersecting with the longitudinal direction of the tubes.  
     
     
         11 . A method for manufacturing a biological substance-immobilized gel microarray, which comprises the following steps: 
 (1) allowing a plurality of hollow tubes to be tied in a bundle;    (2) filling the biological substance-immobilized gel according to any one of  claims 1  to  5  into the hollow space of each tube in the resulting tube bundle; and    (3) cutting the tube bundle in a direction intersecting with the longitudinal direction of the tubes.    
     
     
         12 . A biological substance-immobilized gel microarray which comprises the biological substance-immobilized gel according to any one of  claims 1  to  5 , wherein the gel is arranged in multiple compartments.  
     
     
         13 . The biological substance-immobilized gel microarray according to  claim 12 , wherein the surface area of each compartment is 10 −6  m 2  or less.  
     
     
         14 . The biological substance-immobilized gel microarray according to  claim 12  or  13 , wherein the compartments are formed by slots or through holes.  
     
     
         15 . A biological substance-immobilized gel microarray which is obtained by allowing a plurality of gel-filled hollow tubes according to  claim 8  or  9  to be tied in a bundle and cutting the tube bundle in a direction intersecting with the longitudinal direction of the tubes.  
     
     
         16 . The biological substance-immobilized gel microarray according to  claim 15 , wherein the hollow tubes are hollow fibers.  
     
     
         17 . A method for detecting a target to be measured, which comprises reacting an analyte with the microarray according to any one of  claims 12  to  16  and detecting the target in the analyte.  
     
     
         18 . The method according to  claim 17 , wherein the target to be measured is a nucleic acid.  
     
     
         19 . The method according to  claim 18 , wherein the nucleic acid is 100 nucleotides or less in length.