Sheet for guiding regeneration of mesenchymal tissue and production method thereof

Abstract

To provide a sheet for guiding regeneration of mesenchymal tissue having improved formability, strength, absorbability and efficiency, the sheet is produced by steps of containing a culture medium in a surface of a porous sheet produced by freezing a bioabsorbable polymer material dissolved with an organic solvent and drying it, seeding a mesenchymal cell grown after taking from biotissue, and differentiating the mesenchymal cell to a mesenchymal tissue precursor cell, where the mesenchymal tissue precursor cell and an extracellular substrate are adhered on the surface of the porous sheet containing a culture medium, and the extracellular substrate is secreted in a process of the mesenchymal cell being differentiated to the mesenchymal tissue precursor cell.

1 . A sheet for guiding regeneration of mesenchymal tissue, wherein 
 a mesenchymal precursor cell and an extracellular are adhered on a surface of a porous sheet comprising a bioabsorbable polymer material and containing a culture medium,    the mesenchymal precursor cell is differentiated from a mesenchymal cell taken from biotissue, and the extracellular is secreted in a process in which said mesenchymal cell is differentiated to the mesenchymal precursor cell.    
     
     
         2 . The sheet for guiding regeneration of mesenchymal tissue as claimed in  claim 1 , wherein 
 a bioabsorbable polymer material is at least one kind of material selected from polyglycolic acid, polylactic acid, a copolymer of lactic acid and glycolic acid, poly-ε-caprolactone, a copolymer of lactic acid and ε-caprolactone, polyamino acid, polyortho ester, polymalic acid and a copolymer of those.    
     
     
         3 . The sheet for guiding regeneration of mesenchymal as claimed in  claim 1 , wherein 
 the mesenchymal cell taken from biotissue is a mesenchymal stem cell or a periodontal ligament cell.    
     
     
         4 . The sheet for guiding regeneration of mesenchymal as claimed in  claim 2 , wherein 
 the mesenchymal cell taken from biotissue is a mesenchymal stem cell or a periodontal ligament cell.    
     
     
         5 . The-sheet for guiding regeneration of mesenchymal as claimed in  claim 1 , wherein 
 the mesenchymal precursor cell is any one of a bone blast cell, a cartilage blast cell, a fat blast cell, or a myoblast cell.    
     
     
         6 . The sheet for guiding regeneration of mesenchymal as claimed in  claim 2 , wherein 
 the mesenchymal precursor cell is any one of a bone blast cell, a cartilage blast cell, a fat blast cell, or a myoblast cell.    
     
     
         7 . The sheet for guiding regeneration of mesenchymal as claimed in  claim 3 , wherein 
 the mesenchymal precursor cell is any one of a bone blast cell, a cartilage blast cell, a fat blast cell, or a myoblast cell.    
     
     
         8 . The sheet for guiding regeneration of mesenchymal as claimed in  claim 4 , wherein 
 the mesenchymal precursor cell is any one of a bone blast cell, a cartilage blast cell, a fat blast cell, or a myoblast cell.    
     
     
         9 . A production method of sheet for guiding regeneration of mesenchymal, the method comprising steps of containing a culture medium in the surface of the porous sheet produced by freezing the bioabsorbable polymer material dissolved with an organic solvent and drying it, 
 seeding the mesenchymal cell grown after taking from biotissue, and    differentiating said mesenchymal cell to the mesenchymal precursor cell.    
     
     
         10 . The production method of sheet for guiding regeneration of mesenchymal as claimed in  claim 9 , wherein 
 at least one kind of material selected from polyglycolic acid, polylactic acid, a copolymer of lactic acid and glycolic acid, poly-ε-caprolactone, a copolymer of lactic acid and ε-caprolactone, polyamino acid, polyortho ester, polymalic acid and a copolymer of those, is used as the bioabsorbable high polymer material.    
     
     
         11 . The production method of sheet for guiding regeneration of mesenchymal as claimed in  claim 9 , wherein 
 a mesenchymal stem cell or a periodontal ligament cell is used as the mesenchymal cell taken from biotissue.    
     
     
         12 . The production method of sheet for guiding regeneration of mesenchymal as claimed in  claim 10 , wherein 
 ma mesenchymal stem cell or a periodontal ligament cell is used as the mesenchymal cell taken from biotissue.    
     
     
         13 . The production method of sheet for guiding regeneration of mesenchymal as claimed in  claim 9 , wherein 
 a bone blast cell, a cartilage blast cell, a fat blast cell, or a myoblast cell is used as the mesenchymal tissue precursor cell.    
     
     
         14 . The production method of sheet for guiding regeneration of mesenchymal as claimed in  claim 10 , wherein 
 a bone blast cell, a cartilage blast cell, a fat blast cell, or a myoblast cell is used as the mesenchymal tissue precursor cell.    
     
     
         15 . The production method of sheet for guiding regeneration of mesenchymal as claimed in  claim 11 , wherein 
 a bone blast cell, a cartilage blast cell, a fat blast cell, or a myoblast cell is used as the mesenchymal precursor cell.    
     
     
         16 . The production method of sheet for guiding regeneration of mesenchymal as claimed in  claim 12 , wherein 
 a bone blast cell, a cartilage blast cell, a fat blast cell, or a myoblast cell is used as the mesenchymal precursor cell.