Human lineage committed cell composition with enhanced proliferative potential, biological effector function, or both: methods for obtaining same; and their uses

Abstract

A method for obtaining lineage committed human cells imbued with enhanced proliferative potential, biological function, or both, comprising culturing lineage committed human cells under physiologically acceptable liquid culture conditions, where the liquid culture medium is replaced at a rate and for a time sufficient to obtain the human lineage committed cells imbued with enhanced proliferative potential, biological function, or both; and isolating the cultured cells.

1 - 45 . (canceled)  
   
   
       46 . A composition comprising lineage committed dendritic cells exhibiting enhanced biological function as compared to the biological function of the lineage committed dendritic cells cultured ex vivo under conditions which do not include replacement of the liquid culture medium during the culturing.  
   
   
       47 . The composition of  claim 46 , wherein the biological function enhanced in the isolated lineage committed dendritic cells comprises increased release of cytokines.  
   
   
       48 . The composition of  claim 46 , the lineage committed dendritic cells are cultured under conditions where the liquid culture medium is replaced at a rate of at least 25% daily replacement for more than one day.  
   
   
       49 . The composition of  claim 46 , the lineage committed dendritic cells are cultured under conditions where the liquid culture medium is replaced at a rate of at least 50% daily replacement for more than one day.  
   
   
       50 . The composition of  claim 46 , wherein the lineage committed dendritic cells are cultured under conditions where the liquid culture medium is replaced at a rate of from 25% to 100% daily replacement for about 1×10 4  to about 1×10 7  cells/ml culture for more than one day.  
   
   
       51 . The composition of  claim 46 , wherein the dendritic cells are antigen primed dendritic cells.  
   
   
       52 . The composition of  claim 46 , wherein the dendritic cells are myeloid derived dendritic cells.  
   
   
       53 . The composition of  claim 46 , wherein the dendritic cells are non-myeloid derived dendritic cells.  
   
   
       54 . A composition comprising lineage committed human dendritic cells exhibiting enhanced biological function as compared to the biological function of the lineage committed human dendritic cells cultured ex vivo under conditions which do not include replacement of the liquid culture medium during the culturing.  
   
   
       55 . The composition of  claim 54 , wherein the biological function enhanced in the isolated lineage committed human dendritic cells comprises increased release of cytokines.  
   
   
       56 . The composition of  claim 54 , the lineage committed human dendritic cells are cultured under conditions where the liquid culture medium is replaced at a rate of at least 25% daily replacement for more than one day.  
   
   
       57 . The composition of  claim 54 , the lineage committed human dendritic cells are cultured under conditions where the liquid culture medium is replaced at a rate of at least 50% daily replacement for more than one day.  
   
   
       58 . The composition of  claim 54 , wherein the lineage committed human dendritic cells are cultured under conditions where the liquid culture medium is replaced at a rate of from 25% to 100% daily replacement for about 1×10 4  to about 1×10 7  cells/ml culture for more than one day.  
   
   
       59 . The composition of  claim 54 , wherein the human dendritic cells are antigen primed dendritic cells.  
   
   
       60 . The composition of  claim 54 , wherein the human dendritic cells are myeloid derived dendritic cells.  
   
   
       61 . The composition of  claim 54 , wherein the human dendritic cells are non-myeloid derived dendritic cells.  
   
   
       62 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 46 .  
   
   
       63 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 47 .  
   
   
       64 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 48 .  
   
   
       65 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 49 .  
   
   
       66 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 50 .  
   
   
       67 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 51 .  
   
   
       68 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 52 .  
   
   
       69 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 53 .  
   
   
       70 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 54 .  
   
   
       71 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 55 .  
   
   
       72 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 56 .  
   
   
       73 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 57 .  
   
   
       74 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 58 .  
   
   
       75 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 59 .  
   
   
       76 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 60 .  
   
   
       77 . A method of treating a human patient in need of an infusion of lineage committed human dendritic cells, comprising administering to said patient a composition according to  claim 61.