Synthetic plant genes and method for preparation

Abstract

A method for modifying structural gene sequences to enhance the expression of the protein product is disclosed. Also disclosed are novel structural genes which encode insecticidal proteins of B.t.k. HD-1, B.t.k. HD-73, B.t. tenebrionis , B.t. entomocidus , 2 protein of B.t.k. HD-1, and the coat protein of potato leaf roll virus.

1 .- 38 . (canceled)  
     
     
         39 . A method for minimizing the development of insect resistance to Bt proteins in plants comprising providing a transgenic plant that expresses two Bt insect tolerance proteins with different modes of action.  
     
     
         40 . A plant consisting of two distinct Bt insect tolerance proteins with different modes of action.  
     
     
         41 . Production of two Bt insect tolerance proteins with different modes of action in the same plant.  
     
     
         42 . Expression of two genes encoding Bt insecticidal proteins in the same plant, each with a different mode of action.  
     
     
         43 . Expression of multiple Bt insect tolerance genes in the same plant, said genes having different modes of action.  
     
     
         44 . The genes of  claim 42  wherein said genes lack substantial DNA homology between each other.  
     
     
         45 . The genes of  claim 43  wherein said genes lack substantial DNA homology between each other.  
     
     
         46 . A plant, comprising stably inserted into the genome of its cells, multiple genes encoding different Bt insecticidal proteins each toxic to the same insect species, and each having different modes of action.