Method and solutions for cryopreserving oocytes, especially fresh human oocytes

Abstract

In a method for cryopreserving fresh human oocytes, freezing and thawing solutions are used, which solutions include 1,2 propanediol (PROH) and sucrose at a concentration of at least 0.3M. The oocytes are exposed for 13-15 minutes to a solution including 1.5M PROH and 0.3M sucrose before starting the freezing process.

1 - 11 . (canceled)  
   
   
       12 . A solution for cryopreserving cells, characterized in that it comprises a permeating cryoprotectant and sucrose at a concentration of at least 0.3M.  
   
   
       13 . The solution of  claim 12 , characterized in that the permeating cryoprotectant comprises 1,2-propanediol (PROH) at a concentration of 1.5M and that the solution is adapted for use as a loading solution in cryopreserving processes of oocytes, especially fresh human oocytes.  
   
   
       14 . The solution of  claim 12 , characterized in that the permeating cryoprotectant comprises 1,2 propanediol (PROH) at a concentration of 1.0M and that the solution is adapted for use as a thawing solution in cryopreserving processes of oocytes, especially fresh human oocytes.  
   
   
       15 . The solution of  claim 12 , characterized in that the permeating cryoprotectant comprises 1,2 propanediol (PROH) at a concentration of 0.5M and that the solution is adapted for use as a thawing solution in cryopreserving processes of oocytes, especially fresh human oocytes.  
   
   
       16 . The solution of  claim 12 , characterized in that it is adapted for use as a thawing solution in cryopreserving processes of oocytes, especially fresh human oocytes.