Novel full-length cDNA

Abstract

Novel full-length cDNAs are provided. 2443 cDNA derived from human have been isolated. The full-length nucleotide sequences of the cDNA and amino acid sequences encoded by the nucleotide sequences have been determined. Because the cDNA of the present invention are full-length and contain the translation start site, they provide information useful for analyzing the functions of the polypeptide.

1 - 14 . (canceled)  
     
     
         15 . A polynucleotide selected from the group consisting of the following (a) to (g): (a) a polynucleotide comprising a protein-coding region of the nucleotide sequence of any one of SEQ ID NOs: 1 to 2443; 
 (b) a polynucleotide encoding a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 2444 to 4886;    (c) a polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs: 2444 to 4886, wherein, in said amino acid sequence, one or more amino acids have been substituted, deleted, inserted, and/or added, and wherein said nucleotide sequence encodes a polypeptide functionally equivalent to a polypeptide comprising the selected amino acid sequence;    (d) a polynucleotide hybridizing to a polynucleotide comprising the nucleotide sequence of any one of SEQ ID NOs: 1 to 2443, wherein said nucleotide sequence encodes a polypeptide functionally equivalent to a polypeptide encoded by the selected nucleotide sequence;    (e) a polynucleotide comprising a nucleotide sequence encoding a partial amino acid sequence of a polypeptide encoded by the polynucleotide according to any one or (a) to (d); (f) a polynucleotide comprising a nucleotide sequence having at least 70% identity to the nucleotide sequence of any one of SEQ ID Nos: 1 to 2443; and    (g) a polynucleotide comprising sequences having at least 90% identity to the nucleotide sequence of any one of SEQ ID NOs: 1 to 2443.    
     
     
         16 . A polypeptide encoded by the polynucleotide of  claim 15 , or a partial peptide thereof.  
     
     
         17 . An antibody binding to the polypeptide or the peptide of  claim 16 .  
     
     
         18 . A method for immunologically assaying the polypeptide or the peptide of  claim 16 , said method comprising the steps of contacting the polypeptide or the peptide with the antibody binding to said polypeptide or said peptide, and observing the binding between the two.  
     
     
         19 . A vector comprising the polynucleotide of  claim 15 .  
     
     
         20 . A transformant carrying the polynucleotide of  claim 15  or a vector comprising said polynucleotide.  
     
     
         21 . A transformant carrying the polynucleotide of  claim 15  or a vector comprising said polynucleotide in an expressible manner.  
     
     
         22 . A method for producing a polypeptide or a peptide, said method comprising the steps of culturing the transformant of  claim 21  and recovering an expression product.  
     
     
         23 . An oligonucleotide comprising at least 15 nucleotides, said oligonucleotide comprising a nucleotide sequence complementary to the nucleotide sequence of any one of SEQ ID NOs: 1 to 2443 or to a complementary strand thereof.  
     
     
         24 . A method for synthesizing the polynucleotide of  claim 15 , said method utilizing an oligonucleotide, which comprises a nucleotide sequence complementary to the nucleotide sequence of any one of SEQ ID NOs 1 to 2443 or to a complementary strand thereof, and comprises at least 15 nucleotides.  
     
     
         25 . A method for detecting the polynucleotide of  claim 15 , said method utilizing an oligonucleotide, which comprises a nucleotide sequence complementary to the nucleotide sequence of any one of SEQ ID NOs: 1 to 2443 or to a complementary strand thereof, and comprises at least 15 nucleotides.  
     
     
         26 . An antisense polynucleotide against the polynucleotide of  claim 15  or a part thereof.  
     
     
         27 . A method for detecting the polynucleotide of  claim 15 , said method comprising the following steps of: 
 (a) incubating a target polynucleotide with an oligonucleotice under hybridizable conditions, wherein said oligonucleotide comprises a nucleotide sequence complementary to the nucleotide sequence of any one of SEQ ID NOs: 1 to 2443 or to a complementary strand thereof, and comprises at least 15 nucleotides, and    (b) detecting hybridization of the target polynucleotide with the oligonucleotide.    
     
     
         28 . A database of polynucleotides and/or polypeptides, said database comprising information on at least one of the nucleotide sequences of SEQ ID NOs: 1 to 2443 and/or on at least one of the amino acid sequences of SEQ ID NOs: 2444 to 4886.