US2006105999A1PendingUtilityA1

Novel inhibitors of beta-lactamase

Assignee: METHYLGENE INCPriority: Nov 22, 2002Filed: Nov 19, 2003Published: May 18, 2006
Est. expiryNov 22, 2022(expired)· nominal 20-yr term from priority
C07F 9/6524C07F 9/65312C07F 9/58C07F 9/65586C07F 9/65583C07F 9/425C07F 9/655354C07F 9/65515C07F 9/6541C07F 9/6506C07F 9/4084C07F 9/4006C07F 9/5728C07F 9/4407C07F 9/6539C07F 9/3808C07F 9/65395C07F 9/572C07F 9/65517A61P 31/04C07F 9/655345C07F 9/60C07F 9/2491
43
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Claims

Abstract

The invention relates to bacterial antibiotic resistance and, in particular, to compositions and methods for overcoming bacterial antibiotic resistance. The invention provides novel β-lactamase inhibitors, which are structurally unrelated to the natural product and semi-synthetic β-lactamase inhibitors presently available, and which do not require β-lactam pharmacophore. The invention also provides pharmaceutical compositions and methods for inhibiting bacterial growth.

Claims

exact text as granted — not AI-modified
1 . A compound of the following formula:  
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein 
 R 1  is phenyl or thien-2-yl, each optionally substituted;  
 L is a covalent bond, —CH 2 O—, —C(O)—, or —C(═N—OCH 3 )—; and  
 R 5  is -halo or —OR 10  wherein R 10  is phenyl, pyridinyl, or quinolinyl, each optionally substituted,  
 provided that when L is —CH 2 O—, R 5  is not —F or p-nitrophenyl.  
 
   
   
       2 . The compound according to  claim 1  wherein the substituents are independently selected from —NO 2 , —CO 2 H, and halo.  
   
   
       3 . The compound according to  claim 1  wherein R 1  is unsubstituted.  
   
   
       4 . The compound according to  claim 1  wherein R 5  is selected from:  
     
       
         
         
             
             
         
       
     
   
   
       5 . The compound according to  claim 1  wherein R 1 —L and R 5  are selected from the following combinations:  
     
       
         
               
               
               
             
                   
                   
               
                   
                   
               
                   
                 R 1 —L— 
                 R 5   
               
                   
                   
               
                   
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 PNP 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 PNP 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 PNP 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 PNP 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 
                   
                     
                     
                         
                         
                     
                   
                 
               
                   
                   
               
               
               
               
             
                   
                 and 
                   
               
                   
                   
               
               
               
               
             
                   
                   
                   
               
                   
                 
                   
                     
                     
                         
                         
                     
                   
                 
                 —OH 
               
                   
                   
               
                   
                   
               
           
              
              
              
              
             
             
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
              
             
          
           
              
              
             
          
           
              
              
              
              
             
          
         
       
     
   
   
       6 . The compound according to  claim 1  wherein the phosphonate moiety is replaced with a thiophosphonate moiety, provided that when R 1 —L— is benzyloxy, R 5  is not —O—PNP.  
   
   
       7 . A compound of formula:  
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein 
 R 1  is  
                     
 R 3  is —H or —CO 2 R 9 , wherein R 9  is -C 1 -C 3 -alkyl;  
 R 6  is —L 1 —A—(L 2 —B) s , wherein 
 L 1  is C 0 -C 3 -alkyl optionally mono- to per-halogenated;  
 A is C 3 -C 6 -cycloalkyl, aryl, or heteroaryl;  
 L 2  is a covalent bond or (C 0 -C 3 -hydrocarbyl-X 1 -(C 0 -C 3 -hydrocarbyl), wherein X 1  is —C(O)—, —NH—, —NH—C(O)—, —C(O)—NH—, or heteroaryl;  
 B is —H, C 3 -C 6 -cycloalkyl, aryl, or heteroaryl; and  
 s is 0 or 1;  
 wherein when s is 0, (L 2 —B) s  is —H or halo, and A and B are independently optionally substituted with 1-3 moieties independently selected from the group consisting of halo, —NO 2 , —CO 2 H, —CN, —C(O)—NH 2 , —SO 2 —NH 2 , or -C 0 -C 3 -hydrocarbyl-Y-(C 1 -C 3 -hydrocarbyl) wherein Y is a covalent bond, —O—C(O)—, —C(O)—, —O—, —S—, —SO 2 —, —C(O)—NH—, or —NH—C(O)—; and each alkyl moiety is optionally mono- to per-halogenated.  
 
 
   
   
       8 . The compound according to  claim 7  wherein R 3  is H and R 1  is  
     
       
         
         
             
             
         
       
     
   
   
       9 . The compound according to  claim 7  wherein R 3  is —CO 2 Et and R 1  is  
     
       
         
         
             
             
         
       
     
   
   
       10 . The compound according to  claim 7  wherein L 1  is —O— and A is phenyl or pyridinyl, each optionally substituted, R 3  is H and R 1  is  
     
       
         
         
             
             
         
       
     
   
   
       11 . The compound according to  claim 10  wherein A is pyridin-3-yl.  
   
   
       12 . The compound according to  claim 11  wherein s is 0.  
   
   
       13 . The compound according to  claim 11  wherein s is 1 and L 2  is —C(O)—, —C(O)NH—, —NH—, 1,2,4-oxadiazolyl, or 1,3,4-oxadiazolyl and B is phenyl, pyridinyl, cyclopropyl, or thienyl, wherein B is optionally substituted.  
   
   
       14 . The compound according to  claim 13  wherein the substituents on the A and B rings are independently selected from —F, —Cl, —Br, —CO 2 H, —C(O)O—CH 3 , —CF 3 , —OCH 3 , —OCF 3 , —CH 3 , —CN, —C(O)NH 2 , —S—CF 3 , —SO 2 CH 3 , —NO 2 , —CF 3 CF 3 , —SO 2 CF 3 , —SO 2 CF 3 CF 3 , and —SO 2 NH 2 .  
   
   
       15 . The compound according to  claim 8  wherein one or both of the following are true: 
 a. A is selected from phenyl and pyridinyl;    b. B is selected from phenyl, tetraazolyl, cyclopropyl, pyridinyl, and thienyl.    
   
   
       16 . The compound according to to  claim 9 , wherein R 6  is phenyl or p-nitro phenyl.  
   
   
       17 . The compound according to  claim 8  selected from those in which —O—R 6  is  
     
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
       
         
         
             
             
         
       
     
   
   
       18 . A compound of formula:  
     
       
         
         
             
             
         
       
     
     or a pharmaceutically acceptable salt thereof, wherein 
 R 1  is  
                     
  optionally substituted with 1-3 moieties independently selected from the group consisting of —F, —Cl, —Br, —CO 2 H, —C(O)O—CH 3 , —CF 3 , —OCH 3 , —OCF 3 , -C 1 -C 6  alkyl, —CN, —C(O)NH 2 , —S—CF 3 , —SO 2 CH 3 , —NO 2 , —CF 3 CF 3 , —SO 2 CF 3 , —SO 2 CF 3 CF 3 , and —SO 2 NH 2 ;  
 R 6  is —L 1 —A—(L 2 —B) s , wherein 
 L 1  is C 0 -C 3 -alkyl optionally mono- to per-halogenated;  
 A is C 3 -C 6 -Cycloalkyl, aryl, or heteroaryl;  
 L 2  is a covalent bond or (C 0 -C 3 -hydrocarbyl)-X 1 -(C 0 -C 3 -hydrocarbyl), wherein X 1  is —C(O)—, —NH—, —NH—C(O)—, —C(O)—NH—, or heteroaryl;  
 B is —H, C 3 -C 6 -cycloalkyl, aryl, or heteroaryl; and  
 s is 0 or 1;  
 wherein when s is 0, (L 2 —B) s  is —H or halo, and A and B are independently optionally substituted with 1-3 moieties independently selected from the group consisting of halo, —CF 3 , —NO 2 , —CO 2 H, —CN, —C(O)—NH 2 , —SO 2 —NH 2 , or -C 0 -C 3 -hydrocarbyl-Y-(C 1 -C 3 -hydrocarbyl) wherein Y is a covalent bond, —O—C(O)—, —C(O)—, —O—, —S—, —SO 2 —, —C(O)—NH—, or —NH—C(O)—; and each alkyl moiety is optionally mono- to per-halogenated.  
 
 
   
   
       19 . The compound according to  claim 18  wherein R 6  is phenyl optionally substituted with 1-3 moieties independently selected from the group consisting of halo, —CF 3 , —NO 2 , —CO 2 H, —CN, —C(O)—NH 2 , —SO 2 —NH 2 , or -C 0 -C 3 -hydrocarbyl-Y-(C 1 -C 3 -hydrocarbyl), wherein Y is a covalent bond, —O—C(O)—, —C(O)—, —O—, —S—, —SO 2 —, —C(O)—NH—,or —NH—C(O)—, and each alkyl moiety is optionally mono- to per-halogenated.  
   
   
       20 . The compound according to  claim 19  wherein R 1  is optionally substituted with 1 or 2 moieties independently selected from the group consisting of F, Cl, Br and C 1 -C 6  alkyl.  
   
   
       21 . The compound according to  claim 20  wherein R 1  is  
     
       
         
         
             
             
         
       
     
   
   
       22 . The compound according to  claim 19  wherein R 6  is phenyl optionally substituted with 1 or 2 moieties independently selected from the group consisting of halo, —CF 3 , and CN.  
   
   
       23 . The compound according to  claim 22  wherein the compound is selected from those in which —O—R 6  is;  
     
       
         
         
             
             
         
       
     
   
   
       24 . The compound according to  claim 18  having the structure:  
     
       
         
         
             
             
         
       
     
   
   
       25 . The compound according to  claim 18  having the structure:  
     
       
         
         
             
             
         
       
     
   
   
       26 . The compound according to  claim 18  having the structure:  
     
       
         
         
             
             
         
       
     
   
   
       27 . The compound according to  claim 18  having the structure:  
     
       
         
         
             
             
         
       
     
   
   
       28 . The compound according to  claim 18  having the structure:  
     
       
         
         
             
             
         
       
     
   
   
       29 . The compound according to  claim 18  having the structure:  
     
       
         
         
             
             
         
       
     
   
   
       30 . The compound according to  claim 18  having the structure:  
     
       
         
         
             
             
         
       
     
   
   
       31 . A composition comprising the compound according to  claim 1  and a pharmaceutically acceptable carrier or diluent.  
   
   
       32 . A method of inhibiting β-lactamase, the method comprising contacting a cell with a compound according to  claim 1.

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