Linkage of agents using microparticles

Abstract

Methods, products and kits are provided for attaching agents to a skin surface via microparticles using endogenous or exogenous transglutaminase.

1 . A method of treating a subject to attach microparticles to a skin surface of the subject comprising 
 contacting the skin surface with microparticles having surface available transglutaminase substrate reactive groups in an amount sufficient to attach the microparticles to the skin surface in the presence of endogenous transglutaminase,    allowing the microparticles to remain in contact with the skin surface for a time sufficient to permit a layer of microparticles to covalently attach to the skin surface.    
   
   
       2 . The method of  claim 1 , wherein the surface available transglutaminase substrate reactive groups are lysines.  
   
   
       3 . The method of  claim 1 , wherein the surface available transglutaminase substrate reactive groups are glutamines.  
   
   
       4 . (canceled)  
   
   
       5 . The method of  claim 1 , wherein the microparticles further comprise an active agent.  
   
   
       6 - 8 . (canceled)  
   
   
       9 . The method of  claim 5 , wherein the active agent is not itself a substrate of transglutaminase.  
   
   
       10 - 13 . (canceled)  
   
   
       14 . The method of  claim 1 , wherein the microparticles are 100 nm to 500 nm in size.  
   
   
       15 . The method of  claim 1 , wherein the microparticles are 20 nm to 35 nm in size.  
   
   
       16 - 25 . (canceled)  
   
   
       26 . A method of treating a subject to attach microparticles to a skin surface of the subject comprising 
 contacting the skin surface with microparticles having surface available transglutaminase substrate reactive groups in an amount sufficient to attach the microparticles to the skin surface in the presence of exogenous transglutaminase,    applying exogenous transglutaminase to the skin surface, and    allowing the microparticles and exogenous transglutaminase to remain in contact with the skin surface for a time sufficient to permit a layer of microparticles to covalently attach to the skin surface.    
   
   
       27 . The method of  claim 26 , wherein the surface available transglutaminase substrate reactive groups are lysines.  
   
   
       28 . The method of  claim 26 , wherein the surface available transglutaminase substrate reactive groups are glutamines.  
   
   
       29 . (canceled)  
   
   
       30 . The method of  claim 26 , wherein the microparticles further comprise an active agent  
   
   
       31 - 33 . (canceled)  
   
   
       34 . The method of  claim 30 , wherein the active agent is not itself a substrate of transglutaminase.  
   
   
       35 - 38 . (canceled)  
   
   
       39 . The method of  claim 26 , wherein the microparticles are 100 nm to 500 nm in size.  
   
   
       40 . The method of  claim 26 , wherein the microparticles are 20 nm to 35 nm in size.  
   
   
       41 - 101 . (canceled)  
   
   
       102 . A composition comprising 
 a microparticle comprising an active agent and a lysine-rich or glutamine-rich polymer having transglutaminase substrate reactive groups, wherein the microparticle is non-biodegradable, and the transglutaminase substrate reactive groups are surface available.    
   
   
       103 - 105 . (canceled)  
   
   
       106 . The composition of  claim 102 , wherein the active agent is not itself a substrate of transglutaminase.  
   
   
       107 - 109 . (canceled)  
   
   
       110 . The composition of  claim 102 , wherein the microparticle further comprises a synthetic polymer, and wherein the lysine-rich or glutamine-rich polymer is covalently linked to the synthetic polymer.  
   
   
       111 - 112 . (canceled)  
   
   
       113 . The composition of  claim 102 , wherein the microparticle is 100 nm to 500 nm in size.  
   
   
       114 . The composition of  claim 102 , wherein the microparticle is 20 nm to 35 nm in size.  
   
   
       115 - 116 . (canceled)  
   
   
       117 . The composition of  claim 102 , wherein the transglutaminase substrate reactive groups are surface available in an amount sufficient to attach the microparticle to a skin surface in the presence of endogenous or exogenous transglutaminase.  
   
   
       118 - 145 . (canceled)