Personal care composition containing ozone-stressed yeast lysates

Abstract

The present invention relates to an ozone-stressed yeast lysate, and a personal care composition including an ozone-stressed yeast lysate and at least one preservative selected from the group consisting of alcohols, glycols, parabens, hydantoins, quaternary nitrogen-containing compounds, isothiazolinones, aldehyde-releasing agents, and halogenated compounds. The present invention also relates to a method for preparing and using the ozone-stressed yeast lysate and the personal care composition.

1 . An ozone-stressed yeast lysate produced by a method comprising the steps of: 
 exposing growing yeast to an ozone concentration sufficient to produce ozone-stressed yeast;    lysing the ozone-stressed yeast to produce an ozone-stressed yeast lysate comprising water-soluble and water-insoluble components; and    separating the water-soluble components from the water-insoluble components to produce an ozone-stressed yeast lysate comprising the water-soluble components.    
   
   
       2 . An ozone-stressed yeast lysate produced by method according to  claim 1 , wherein the ozone concentration is between about 0.0001 millimole (mM) and about 1.0 millimole (mM).  
   
   
       3 . An ozone-stressed yeast lysate produced by a method according to  claim 1 , wherein the yeast is lysed by at least one enzyme, high-speed agitation, autolysis or a change in pH.  
   
   
       4 . A personal care composition comprising: 
 an ozone-stressed yeast lysate; and    a preservative,    wherein the preservative is selected from the group consisting of alcohol, glycol, parabens, hydantoins, quaternary nitrogen-containing compounds, isothiazolinones, aldehyde-releasing agents, halogenated compounds, and combinations thereof.    
   
   
       5 . A personal care composition of  claim 4 , wherein the ozone-stressed yeast lysate is present in a concentration between about 0.0001% and about 100% by weight based on the total weight of the personal care composition.  
   
   
       6 . A personal care composition of  claim 5 , wherein the ozone-stressed yeast lysate is present in a concentration between about 0.01% and about 50% by weight based on the total weight of the personal care composition.  
   
   
       7 . A personal care composition of  claim 6 , wherein the ozone-stressed yeast lysate is present in a concentration between about 1% to about 10% by weight based on the total weight of the personal care composition.  
   
   
       8 . A personal care composition of  claim 4 , wherein the preservative is present in a concentration between about 0.01% to about 10% by weight based on the total weight of the personal care composition.  
   
   
       9 . A personal care composition of  claim 4 , wherein the ozone-stressed yeast lysate is encapsulated with another component to provide a time-release characteristic to the ozone-stressed yeast lysate.  
   
   
       10 . A personal care composition of  claim 9 , wherein the other component is selected from the group consisting of lyposomes, niosomes, sub-micron emulsions, polymeric encapsulants, gels, creams, lotions, and combinations thereof.  
   
   
       11 . A personal care composition according to  claim 4 , wherein the ozone-stressed yeast lysate comprises at least one cellular component selected from the group consisting of cellular protein material, cellular nuclear material, cellular cytoplasmic material, cellular protoplasmic material, cell wall components, and combinations thereof.  
   
   
       12 . A personal care composition according to  claim 4 , wherein the ozone-stressed yeast lysate is essentially water-soluble.  
   
   
       13 . A composition for topical treatment of skin, comprising the ozone-stressed yeast lysate of  claim 1  and a cosmetically acceptable vehicle for application to the skin.  
   
   
       14 . A method for preparing a personal care composition comprising an ozone-stressed yeast lysate effective in reducing or minimizing ozone-induced skin degradation when applied to skin cells, the method comprising the steps of: 
 growing yeast in a fermentation broth;    exposing the yeast to ozone by aerating the yeast for a time period of between about five minutes and about seventy-two hours with an aeration gas having an ozone concentration of from about 0.0001 millimole (mM) to about 1.0 millimole (mM) based on the total volume of the fermentation broth to produce ozone-stressed yeast;    lysing the ozone-stressed yeast to produce an ozone-stressed yeast lysate; and    incorporating the ozone-stressed yeast lysate into a personal care composition.    
   
   
       15 . A method for preparing a personal care composition according to  claim 14  wherein the aeration gas has a temperature between about 15° C. and about 90° C.  
   
   
       16 . A method for preparing a personal care composition according to  claim 14 , wherein the yeast is aerated for a time period between about 10 minutes and about 60 minutes with an aeration gas having an ozone concentration of from about 0.01 mM to about 0.8 mM.  
   
   
       17 . A method for preparing a personal care composition according to  claim 16 , wherein the yeast is aerated for a time period between about 15 minutes and about 30 minutes with an aeration gas having an ozone concentration of from about 0.1 mM to about 0.5 mM.  
   
   
       18 . A method for preparing a personal care composition according to  claim 14 , wherein the personal care composition further comprises at least one ingredient selected from the group consisting of water, surfactants, emulsifiers, conditioners, emollients, waxes, oils, polymers, thickeners, fixatives, colorants, humectants, moisturizers, stabilizers, diluents, solvents, fragrance, botanicals, neutraceuticals, cosmeceuticals, antifungals, antimicrobials, steroidal hormones, antidandruff agents, anti-acne components, sunscreen, preservatives, and combinations thereof.